Molecular and phylogenetic analysis of MADS-box genes of MIKC type and chromosome location of SEP-lke genes in n wheat (Triticum aestivum L.).
Author(s)
Paolacci, Anna Rita
Tanzarella, Oronzo A.
Porceddu, Enrico
Varotto, Serena
Ciaffi, Mario
Date Issued
2007
Type
Article
Abstract
Transcription factors encoded by MIKC-type
MADS-box genes control many important functions in
plants, including Xower development and morphogenesis.
The cloning and characterization of 45 MIKC-type MADSbox
full-length cDNA sequences of common wheat is
reported in the present paper. Wheat EST databases were
searched by known sequences of MIKC-type genes and
primers were designed for cDNA cloning by RT-PCR.
Full-length cDNAs were obtained by 5 and 3 RACE
extension. Southern analysis showed that three copies of
the MIKC sequences, corresponding to the three homoeologous
genes, were present. This genome organization was
further conWrmed by aneuploid analysis of six SEP-like
genes, each showing three copies located in diVerent homoeologous
chromosomes. Phylogenetic analysis included the
wheat MIKC cDNAs into 11 of the 13 MIKC subclasses
identiWed in plants and corresponding to most genes controlling
the Xoral homeotic functions. The expression patterns
of the cDNAs corresponding to diVerent homeotic
classes was analysed in 18 wheat tissues and Xoral organs
by RT-PCR, real time RT-PCR and northern hybridisation.
Additional information
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Citation
Paolacci A.R., et al. 2007. Molecular and phylogenetic analysis of MADS-box genes of MIKC type and chromosome location of SEP-lke genes in n wheat (Triticum aestivum L.). "Mol Genet Genomics" 278: 689-708
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