A candidate for Lr19, an exotic gene conditioning leaf rust resistance in wheat
Author(s)
Gennaro, Andrea
Koebner, Robert M.D.
Ceoloni, Carla
Date Issued
2009
Type
Article
Abstract
Lr19, one of the few widely effective genes conferring resistance to leaf-rust in wheat, was transferred from the wild relative Thinopyrum ponticum to durum wheat. Since Lr19 confers a hypersensitive response to the pathogen, it was considered likely that the gene would be a member of the major nucleotide-binding site (NBS)-leucine-rich repeat (LRR) plant R gene family. NBS profiling, based on PCR amplification of conserved NBS motifs, was applied to durum wheat-Thinopyrum ponticum recombinant lines involving different segments of the alien 7AgL chromosome arm, carrying or lacking Lr19.
One sequence (AG15), tightly linked to Lr19, a 4,121 bp full-length cDNA was obtained. Quantitative PCR on leaf-rust infected and non-infected Lr19 carriers proved AG15 to be constitutively expressed, as is common for R genes.
Additional information
L'articolo รจ disponibile sul sito dell'editore: http://www.springerlink.com.
Citation
Gennaro A., Koebner R.M.D., Ceoloni C. 2009. A candidate for Lr19, an exotic gene conditioning leaf rust resistance in wheat. "Functional and Integrative Genomics" 9(3): 325-334.
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