Novel Alleles of the Potato Leaf Gene Identified in Italian Traditional Varieties Conferring Potato-like Leaf Shape in Tomato
Author(s)
Date Issued
January 25, 2025
Type
article
Volume
11
Issue
2
ISSN
2311-7524
Journal
Abstract
The genetic diversity of tomato in Italy and the growing interest in high-quality
food products highlight the importance of establishing varietal distinctiveness through molecular
strategies to ensure agrifood product quality and traceability. In this study, four Italian
potato-like leaf (PL) landraces were analyzed: “Spagnoletta di Formia e di Gaeta” (SPA)
from southern Lazio, “Giagiù” (GIA) and “Patanara” (PTN) from Campania, and “Pomodoro
di Mola” (MOL) from Apulia. These landraces were genotyped for the potato leaf gene
(C), with two PL American genotypes and a non-allelic PL mutant line included as outgroups.
Nagcarlang served as control. An allelism test confirmed C as determinant gene.
The SCAR marker for C revealed that the Italian landraces presented determinants other
than the most representative one responsible for PL. Whole-genome sequencing of SPA
identified a private novel nonsense SNP variant allele, confirmed through dCAPS marker
analysis. Additionally, two novel PL alleles responsible for missense variations were identified
in GIA/PTN and MOL. In silico protein analysis suggested that novel C alleles could
be functional determinants for the protein activity. Overall, PL mutations identified for the
first time could serve as molecular tools for agrifood chain traceability, enabling early differentiation
and recognition of genotypically similar varieties.
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