Effects of administration of probiotic strains on GALT of larval gilthead seabream: immunohistochemical and ultrastructural studies
Author(s)
Date Issued
2007
Type
Article
Abstract
Two bacterial strains Lactobacillus fructivorans (AS17B), isolated from adult seabream (Sparus aurata L.) gut, and Lactobacillus
plantarum (906), isolated from human faeces, were administered contemporaneously during seabream development using
Brachionus plicatilis and/or Artemia salina and dry feed as vectors.
Experimental group A received the probiotic strains already via rotifers from day 5 post-hatch (ph), whereas treatment of group
B began with Artemia feeding from day 27 ph. Fish were sampled at day 28 ph (group A and control) and day 99 ph (groups A, B
and control) for electron microscopy, histology and immunohistochemistry with the polyclonal antiserum ORa against homologous
serum Ig and the mAb G7 specific for seabream acidophilic granulocytes. In all groups, timing and pattern of differentiation of the
digestive tract did not differ. Furthermore, neither tissue damage nor manifest inflammation was provoked by probiotic administration.
At day 28 ph, the developing GALTalready housed mucosal leucocytes, including Igþ cells but no acidophilic granulocytes. No
differences were seen between experimental groups.
At day 99 ph, the density of Igþ cells (þ51%) and acidophilic granulocytes (þ284%) was significantly higher (p < 0.05) in
group A than in controls. Also group B had a higher density of Igþ cells (þ17%) and acidophilic granulocytes (þ130%) compared
with controls, although less pronounced. Light and electron microscopy observations detailed the occurrence of heterogeneous populations
of lymphocytes and granulocytes in the developing intestinal mucosa, and highlighted the net expansion of G7þ acidophilic
granulocytes (A þ536%, B þ292% vs. control) due to probiotic administration.
Additional information
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Citation
Picchietti, S. et al. 2007. Effects of administration of probiotic strains on GALT of larval gilthead seabream: Immunohistochemical and ultrastructural studies. "Fish and Shellfish Immunology" 22(1): 57-67
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