Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3/HP1/Me(3)K20H4 pathway
Author(s)
Date Issued
2007
Type
Article
Abstract
Using RNA interference (RNAi) we have conducted a functional analysis of the HP1-like chromobox gene pchet2 during embryogenesis of the mealybug Planococcus citri.
Knocking down pchet2 expression results in
decondensation of the male-specific chromocenter that normally arises from the developmentally-regulated facultative heterochromatinisation of the paternal chromosome complement. Together with the disappearance of the chromocenter the staining levels of two associated histone modifications, tri-methylated lysine 9 of histone H3 [Me(3)K9H3] and tri-methylated lysine 20 of histone H4 [Me(3)K20H4], are reduced to undetectable levels. Embryos treated with double-stranded RNA (dsRNA) targeting pchet2 also exhibit chromosome abnormalities, such as aberrant chromosome condensation, and also the presence of metaphases that contain ‘lagging’ chromosomes. We conclude that PCHET2 regulates chromosome behavior during metaphase and is a crucial component of a Me(3)K9H3-HP1-Me(3)K20H4 pathway involved in the facultative heterochromatinisation of the (imprinted) paternal chromosome set.
Additional information
L'articolo è disponibile sul sito dell'editore: http://jcs.biologists.org.
Citation
Bongiorni, S. et al. 2007. Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3/HP1/Me(3)K20H4 pathway. "Journal of Cell Science" 120(6): 1072-1080.
File(s)![Thumbnail Image]()
Name
Abstract.doc
Size
21 KB
Format
Microsoft Word
Checksum (MD5)
707b394924b3db0b8a5e4bd53bb2e70c