Effect of storage conditions of blood on radiation-induced chromosomal aberrations and apoptosis in human lymphocytes
Author(s)
Belloni, Paola
Pepe, Gaetano
Palitti, Fabrizio
Date Issued
2010
Type
Article
Abstract
To evaluate the effect of storage conditions of blood on the direct relationship between radiation-induced
chromosome aberrations and apoptosis in human peripheral blood lymphocytes,whole bloodwas irradi-
atedwith 3Gy X-rays. Directly after irradiation, a sample of bloodwas analyzed for chromosome damage
and proliferation index, after phytohaemagglutinin stimulation and incubation at 37 ◦
C for 56 h. Blood
samples were stored for 48 h at 4 and 20 ◦
C with or without phytohaemagglutinin and analyzed for
cell viability and apoptosis at 0, 24 and 48 h storage time. After 48 h of storage, unstimulated cultures
were stimulated to proliferate. These samples and cultures stimulated immediately before storage were
incubated at 37 ◦
C for 56 h and analyzed for chromosome damage and proliferation index. Metaphases
were examined for the presence of dicentrics, excess acentrics, and rings. Storage at 20 ◦
Cwithout phyto-
haemagglutinin for 48 h increases significantly the yield of apoptosis and decreases significantly the yield
of dicentrics. During 48 h of storage time the presence of phytohaemagglutinin and the temperature of
4 ◦
C protected the irradiated lymphocytes from apoptosis allowing accurate estimation of the real yield
of radiation-induced chromosome damage. Therefore these blood-storage conditions enable analysis in
metaphase and may offer some advantages for biodosimetry of absorbed radiation dose.
Additional information
L'articolo è disponibile sul sito dell'editore: http://www.sciencedirect.com
Citation
Belloni P. Pepe G. Palitti F. 2010. Effect of storage conditions of blood on radiation-induced chromosomal aberrations and apoptosis in human lymphocytes. "Mutation Research" 695(1-2): 35-39
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