Analysis of the expression and modulation of selected immune-related gene transcripts in the DLEC cell line from European sea bass (Dicentrarchus labrax)
Author(s)
Buonocore, Francesco
Randelli, Elisa
Lorenzen, Niels
Einer-Jensen, Katja
Scapigliati, Giuseppe
Date Issued
2011
Type
Article
Abstract
Cell lines have been established from different fish species especially for virus isolation and for studying cell-pathogen
interactions, and therefore are of interest in aquaculture. In this paper, we have investigated the presence and the
regulation of some immune genes in the DLEC (Dicentrarchus labrax embryonic cells) cell line from European sea bass
(Dicentrarchus labrax L.) to preliminary elucidate their action. The basal expression of the selected genes (interleukin-
1β (IL-1β), cyclooxygenase-2 (COX-2), transforming growth factor-β (TGF-β), CD8-α, major histocompatibility complex
II-β (MHC II-β), interferon (IFN) and Mx protein (Mx)) have been investigated and, successively, their modulation have
been studied both after stimulation with different mitogen agents and after a transfection with a sequence codifying for
the coat protein of a fish nervous necrosis virus (NNV). The results have evidenced that the inflammatory molecules
(IL-1β, COX-2, TGF-β), constitutively expressed by the DLEC cell line, are not up-regulated by the stimulation with
lipopolysaccharide (LPS) from E. coli, whether the expression of the T-cell marker transcripts (CD8-α, MHC II-β) is
influenced by the action of a lectin from Phaseolus vulgaris (PHA-L). Finally, the expression of the coat NNV protein
in the DLEC cell line, after the transfection, led to an high up-regulation of IFN and Mx gene transcripts. These data
suggest that the DLEC cell line recognize specific pathogen-associated molecular patterns (PAMPs) and, therefore,
could be useful for studying T-cell pathways and viral responses in sea bass avoiding the use of live test animals.
Citation
Buonocore, F. et al. 2011. Analysis of the expression and modulation of selected immune-related gene transcripts in the DLEC cell line from European sea bass (Dicentrarchus labrax) "Journal of Aquaculture Research & Development" 2 (1): 105
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