"Survey on Xanthomonas campestris pv. campestris strains from Brassica spp. in Latium region", L.Albanese, D. Schiavi, G.M. Balestra. XXX SIPaV Congress, Catania Monastero dei Benedettini, 15-17 Settembre 2025.
Author(s)
Leonardo Albanese
Date Issued
2025
Type
conferenceObject
Volume
Volume 107
Start Page
1575
End Page
1702
Abstract
Black rot of cabbage, caused by Xanthomonas campestris pv. campestris (Xcc), is one of the most pervasive diseases of Brassica spp. worldwide. Symptoms can be observed on leaf presenting V-shaped necrosis on major veins that can progress through the inflorescence and the corymb. Control techniques nowadays rely mostly on the use of copper which could have been caused the emergence of resistance within the bacterial populations. The aim of the work was to collect and characterize wild strains of Xcc from different farms with relevant cabbage crops in Northern Latium Region and screen them for copper sensitivity. Samples were firstly isolated from diseased leaves of different Brassica species. Several Xcc strains were obtained and 79 tested positive for the colony PCR assay with specific primers. Subsequently, a in vivo test was run to assess the pathogenicity of the isolated strains by inoculating them on seedlings of Brassica oleracea L. convar. botrytis L. var. Italica cv. Naxos; at the same time, the strains were screened to verify the amylolytic activity in a starch-composed media and the copper tolerance on nutrient agar amended with two different CuSO4 concentrations (100 ppm and 200 ppm).
Confirmed Xcc strains provoked the expected symptoms at 7-10 days post-inoculation. All the selected strains tested positive for the amylolytic activity; all the strains were able to grow on copper media at 100 ppm and only 7 strains did not grow at 200 ppm, indicating a potential threat to the effectiveness and the sustainability of the current protection strategies based on copper.
Conference(s)
XXX CONGRESS OF THE ITALIAN PHYTOPATHOLOGICAL SOCIETY Plant Health: Contributions of Plant Pathology To a Sustainable Future. Catania September 15-17, 2025 Monastero dei Benedettini