Optimization of the growth conditions through response surface methodology and metabolomics for maximizing the auxin production by Pantoea agglomerans C1
Author(s)
Date Issued
2023
Type
article
Volume
14
Start Page
1
End Page
14
Journal
Abstract
Array of compounds with plant growth-promoting-
(IAA and hypoxanthine) and biocontrol activity (NS-5, cyclohexanone, homo-Larginine,
methyl hexadecenoic acid, and indole-3-carbinol) can be stimulated
by cultivating this strain on minimal saline medium amended with sucrose as a
carbon source. We applied a three-level-two-factor central composite design
(CCD) based response surface methodology (RSM) to explore the impact of
the independent variables (rotation speed and medium liquid-to-flask volume
ratio) on the production of IAA and IAA precursors. The ANOVA component
of the CCD indicated that all the process-independent variables investigated
significantly impacted the auxin/IAA production by P. agglomerans strain C1. The
optimum values of variables were a rotation speed of 180 rpm and a medium
liquid-to-flask volume ratio of 1:10. Using the CCD-RSM method, we obtained
a maximum indole auxin production of 208.3 ± 0.4 mg IAAequ/L, which was a 40%
increase compared to the growth conditions used in previous studies. Targeted
metabolomics allowed us to demonstrate that the IAA product selectivity and
the accumulation of the IAA precursor indole-3-pyruvic acid were significantly
affected by the increase in the rotation speed and the aeration efficiency
Subjects