Redesigning the reactive site loop of the wheat subtilisin/chymotrypsin inhibitor (WSCI) by site-directed mutagenesis. A protein–protein interaction study by affinity chromatography and molecular modeling
Author(s)
Date Issued
2009
Type
Article
Abstract
A site-directed mutagenesis strategy was employed to obtain four mutants of wheat subtilisin/chymotrypsin inhibitor (WSCI), with the aim to produce inactive forms of this protein. The mutants were expressed in Escherichia coli as fusion proteins and, after the tag removal, were purified to homogeneity and characterized, for their interaction with the inhibited enzymes, by affinity chromatography and molecular modeling.
Additional information
L'articolo é disponibile sul sito dell'editore: http://www.sciencedirect.com
Citation
Bruni, N. et al. 2009. Redesigning the reactive site loop of the wheat subtilisin/chymotrypsin inhibitor (WSCI) by site-directed mutagenesis. A protein–protein interaction study by affinity chromatography and molecular modeling. "Biochimie". 91, 1112-1122.
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336146a02cd66d87045c6a6a96f7452c
Sponsor(s)
1. Grant COFIN 2006 by Ministero Italiano Istruzione Università e Ricerca (MIUR) and Università degli Studi della Tuscia. 2. Seconda Università degli Studi di Napoli. 3. Post-doc felloship by Università degli Studi della Tuscia