The involvement of chromatin condensation in campthotecin-induced chromosome breaks in G0 human lymphocytes
Author(s)
Date Issued
1999
Type
Article
Abstract
In the present study we evaluated campthotecin (CPT)-induced chromosomal damage in human lymphocytes in the G0 phase of the cell cycle as revealed by the premature chromosome condensation technique. The results obtained here indicate that CPT was able to induce chromosome fragments in the G0 phase of the cell cycle of human lymphocytes as detected in prematurely condensed chromosomes. This result appears to be rather surprising, since the DNA lesions produced by CPT (e.g. 'protein concealed' DNA single-strand breaks) should not produce any damage in G0. A possible explanation for this result could come from much evidence to suggest that chromatin condensation processes are significantly involved in the conversion of DNA lesions into chromosome breaks in prematurely condensed chromosomes. The unexpected clastogenic behaviour of CPT can be explained taking into account the chromosome condensation induced by mitosis promoting factors when human lymphocytes are fused in G0, thus converting the 'protein concealed' DNA single-strand breaks induced by CPT into chromosome breaks. The same perspective should be taken into consideration for breaks induced by CPT under normal physiological conditions in the G2 phase of the cell cycle.
Additional information
L'articolo é disponibile sul sito dell'editore: http://www.oxfordjournals.org
Citation
Mosesso, P. et al. 1999. The involvement of chromatin condensation in campthotecin-induced chromosome breaks in G0 human lymphocytes. "Mutagenesis" 14(1): 103-105.
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