Caratterizzazione di metaboliti secondari presenti in varie specie di Cistus (fam. Cistaceae) in Sardegna
Author(s)
Mastino, Patrizia Monica
Date Issued
May 3, 2017
Type
Doctoral Thesis
Abstract
Cistus genus is present in Sardinia with populations of C. monspeliensis, C. salvifolius, C. creticus
subsp. eriocephalus, C. albidus, C. creticus subsp. creticus and C. creticus subsp. corsicus. No
chemical studies are currently being carried on Cistus species of Sardinia. The aim of this research
is to characterize the secondary metabolites in extracts of different species of Cistus in Sardinia and
to evaluate antimicrobial activity. The plants were extracted using hydro-distillation and several
traditional solvents and were characterized using GC/MS, HPLC-DAD-ESI-MS and CL-UHPSM/
SM. Antimicrobial activity was determined as MIC by using an agar macrodilution method.
The essential oils have shown six different characteristic profiles. The profiles of the three C.
creticus subspecies are similar, even if the relative percentage are quite different. The analysis of the
seven essential oils of C. c. eriocephalus show interesting characteristics and they would divided in
groups with different metabolic profiles. Among solvent extracts the obtained results allowed the
detection of several phenolic compounds.They are characterized by a hight percentage of
rosmarinic acid derivatives and of quercitin derivatives. C. salvifolius is quantitatively most rich of
phenolic compounds. The extracts exhibited any differences in their antimicrobial activities: Grampositive
bacteria are more sensitive to the Cistus extracts. Any noticeable action has been found
against Candida species.
Additional information
Dottorato di ricerca in Biotecnologie vegetali
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Sponsor(s)
In co-tutela di tesi con:
Università di Corsica-Pasquale Paoli, Ecole Doctorale "Environnement et Société"- UMR CNRS 6134 SPE
Università di Corsica-Pasquale Paoli, Ecole Doctorale "Environnement et Société"- UMR CNRS 6134 SPE