Identification of chromosome 5A encoded polypeptides in wheat kernels
Author(s)
Gombaud, Gaëtan
Date Issued
April 20, 2012
Type
Doctoral Thesis
Abstract
This PhD work has been performed in the frame of the Italian project “Physical map of
wheat chromosome 5A: Italian initiative for the sequencing of the whole genome”, that is part
of the international initiatives ETGI & IWSC.
Wheat kernel proteins are represented for 80% of gluten and for 20% by soluble proteins.
Whereas chromosome localization of the former is well known, that of soluble proteins,
including polypeptides with structural and metabolic functions, still needs to be identified and
mapped.
A proteomic approach has been performed to identify polypeptides encoded by genes on
chromosome 5A. Intervarietal and interspecific chromosome substitution lines of durum and
bread wheat have been used, leading to the identification of 48 soluble proteins in the two
tetraploid genotypes taken into consideration, and a total of 86 proteins in the 5 bread wheat
cultivars analysed.
The biological functions of most of the identified polypeptides are: stress/defense;
carbohydrate metabolism, protein synthesis/assembly, storage, and a significative portion
corresponds to unknown proteins.
The knowledge of polypeptides encoded by genes at chromosomes 5A will allow
correlating their presence with specific physiological characteristics, along with quality
properties.
Additional information
Dottorato di ricerca in Biotecnologie vegetali
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