Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion
Author(s)
Date Issued
2010
Type
Article
Abstract
Plant cell walls represent an abundant, renewable source of biofuels and other useful products. The major bottleneck for the industrial scale-up of their conversion to simple sugars (saccharification) is their recalcitrance to enzymatic hydrolysis. We demonstrated that the structure of pectin affects the exposure of cellulose to enzymes and consequently the process of saccharification.
Le pareti cellulari vegetali rappresentano una abbondante e rinnovabile sorgente di biocarburanti ed altri prodotti utili. Una delle maggiori difficoltà nell’utilizzo delle pareti cellulari a livello industriale è la loro conversione in zuccheri semplici (saccarificazione) in quanto sono estremamente resistenti all’idrolisi enzimatica. In questo lavoro dimostriamo che la struttura della pectina influenza l’esposizione della cellulosa all’azione enzimatica e conseguentemente il processo di saccarificazione.
Citation
Lionetti, V. et al. 2010. Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion. "PNAS" 107(2): 616-621.
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Sponsor(s)
This work was supported by the European Research Council [ERC Advanced Grant 233083 (F. C.)], the Institute Pasteur-Fondazione Cenci Bolognetti, the Italian Ministry of University and Research [PRIN 2007 Grant (G. D. L., F.C. and R.D) and AgroGen Grant (R.D.)] and the European Union COST Action 928 (D.B.).