La sovraespressione della PGIP in piante transgeniche di frumento determina un incremento di resistenza contro i patogeni fungini
Author(s)
Janni, Michela
Date Issued
March 15, 2006
Type
Doctoral Thesis
Abstract
A possible strategy to control plant pathogens is the improvement of natural plant defence mechanisms against the processes that pathogens commonly use to penetrate and colonize the host tissue. One of these defence mechanisms is the ability to inhibit the pathogen’s capability to degrade plant cell walls. Polygalacturonase-inhibiting proteins (PGIPs) are plant defence glycoproteins associated with the cell wall of both monocot and dicot species. They interact with fungal endopolygalacturonases (PGs) and modulate their activity favouring the accumulation of oligogalacturonides active as elicitors of plant defence responses.
To assess the effectiveness of these proteins in protecting wheat from the fungal pathogens, we have produced a number of transgenic wheat lines expressing a bean PGIP (PvPGIP2) having a wide spectrum of specificities against fungal PGs. Six of them have been selected and tested to value PvPGIP2 expression level and inhibition activity. Results shows that the transgene-encoded protein is correctly secreted in the apoplast, maintains the characteristic recognition specificities and endows the transgenic wheats with new PG recognition capabilities. As a consequence, transgenic wheat tissue showed an increased resistance to Fusarum moniliforme PG’s digestion. Three transgenic lines (J82-23a, J82-200, MJ7-4) over expressing PvPGIP2 showed a significant reduced symptom progression through the leaves or spikes following infection with Bipolaris sorokiniana and in one transgenic line (J82-23a), the accumulation of PvPGIP2 confers partial protection against the spread of F. graminearum in inoculated wheat spikes.
Additional information
Dottorato di ricerca in Biotecnologie vegetali
Subjects
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