Asynchronously replicating Eu/heterochromatic regi... [Cytogenet Genome Res. 2010] - PubMed result Resources All ResourcesChemicals & Bioassays BioSystemsPubChem BioAssayPubChem CompoundPubChem Structure SearchPubChem SubstanceAll Chemicals & Bioassays Resources…DNA & RNA BLAST (Basic Local Alignment Search Tool)BLAST (Stand-alone)E-UtilitiesGenBankGenBank: BankItGenBank: SequinGenBank: tbl2asnGenome WorkbenchInfluenza VirusNucleotide DatabasePopSetPrimer-BLASTProSplignReference Sequence (RefSeq)RefSeqGeneSequence Read Archive (SRA)SplignTrace ArchiveUniGeneUniSTSAll DNA & RNA Resources…Data & Software BLAST (Basic Local Alignment Search Tool)BLAST (Stand-alone)Cn3DConserved Domain Search Service (CD Search)E-UtilitiesGenBank: BankItGenBank: SequinGenBank: tbl2asnGenome ProtMapGenome WorkbenchPrimer-BLASTProSplignPubChem Structure SearchSNP Submission ToolSplignVector Alignment Search Tool (VAST)All Data & Software Resources…Domains & Structures BioSystemsCn3DConserved Domain Database (CDD)Conserved Domain Search Service (CD Search)Structure (Molecular Modeling Database)Vector Alignment Search Tool (VAST)All Domains & Structures Resources…Genes & Expression BioSystemsDatabase of Genotypes and Phenotypes (dbGaP)E-UtilitiesGeneGene Expression Omnibus (GEO) Database Gene Expression Omnibus (GEO) DatasetsGene Expression Omnibus (GEO) ProfilesGenome WorkbenchHomoloGeneMap ViewerOnline Mendelian Inheritance in Man (OMIM)RefSeqGeneUniGeneAll Genes & Expression Resources…Genetics & Medicine BookshelfDatabase of Genotypes and Phenotypes (dbGaP)Influenza VirusMap ViewerOnline Mendelian Inheritance in Man (OMIM)PubMedPubMed Central (PMC)PubMed Clinical QueriesRefSeqGeneAll Genetics & Medicine Resources…Genomes & Maps Database of Genomic Structural Variation (dbVar)GenBank: tbl2asnGenomeGenome ProjectGenome ProtMapGenome WorkbenchInfluenza VirusMap ViewerNucleotide DatabasePopSetProSplignSequence Read Archive (SRA)SplignTrace ArchiveUniSTSAll Genomes & Maps Resources…Homology BLAST (Basic Local Alignment Search Tool)BLAST (Stand-alone)BLAST Link (BLink)Conserved Domain Database (CDD)Conserved Domain Search Service (CD Search)Genome ProtMapHomoloGeneProtein ClustersAll Homology Resources…Literature BookshelfE-UtilitiesJournals in NCBI DatabasesMeSH DatabaseNCBI HandbookNCBI Help ManualNCBI NewsPubMedPubMed Central (PMC)PubMed Clinical QueriesAll Literature Resources…Proteins BioSystemsBLAST (Basic Local Alignment Search Tool)BLAST (Stand-alone)BLAST Link (BLink)Conserved Domain Database (CDD)Conserved Domain Search Service (CD Search)E-UtilitiesProSplignProtein ClustersProtein DatabaseReference Sequence (RefSeq)All Proteins Resources…Sequence Analysis BLAST (Basic Local Alignment Search Tool)BLAST (Stand-alone)BLAST Link (BLink)Conserved Domain Search Service (CD Search)Genome ProtMapGenome WorkbenchInfluenza VirusPrimer-BLASTProSplignSplignAll Sequence Analysis Resources…Taxonomy TaxonomyTaxonomy BrowserTaxonomy Common TreeAll Taxonomy Resources…Training & Tutorials NCBI Education PageNCBI HandbookNCBI Help ManualNCBI NewsScience PrimerAll Training & Tutorials Resources…Variation Database of Genomic Structural Variation (dbVar)Database of Genotypes and Phenotypes (dbGaP)Database of Single Nucleotide Polymorphisms (dbSNP)SNP Submission ToolAll Variation Resources…How To All How ToChemicals & BioassaysDNA & RNAData & SoftwareDomains & StructuresGenes & ExpressionGenetics & MedicineGenomes & MapsHomologyLiteratureProteinsSequence AnalysisTaxonomyTraining & TutorialsVariationSkip to main contentSkip to navigationAbout NCBI AccesskeysMy NCBISign InSign OutPubMedU.S. National Library of Medicine National Institutes of Health Search:All DatabasesPubMedProteinNucleotideGSSESTStructureGenomeBioSampleBioSystemsBooksCancerChromosomesConserved DomainsdbGaPdbVarEpigenomicsGeneGenome ProjectGENSATGEO DataSetsGEO ProfilesHomoloGeneImagesMeSHNCBI Web SiteNLM CatalogOMIAOMIMPMCPopSetProbeProtein ClustersPubChem BioAssayPubChem CompoundPubChem SubstanceSNPSRATaxonomyToolKitToolKitAllUniGeneUniSTSSearchClearLimitsAdvanced searchHelp Display Settings:AbstractFormat SummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListApplySend to:Choose DestinationFileClipboardCollectionsE-mailOrderMy Bibliography Format Summary (text)Abstract (text)MEDLINEXMLPMID ListCSV Create File 1 selected item: 20407222 Format SummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListMeSH and Other Data E-mail Additional text E-mail "SPAM" filtering software notice Add to Clipboard Add to Collections Order articles Add to My Bibliography Cytogenet Genome Res. 2010;128(1-3):111-7. Epub 2010 Apr 20. Asynchronously replicating Eu/heterochromatic regions shape chromosome damage. Di Tomaso MV, Martínez-López W, Palitti F. Department of Genetics, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, Uruguay. marvi@iibce.edu.uy Abstract In order to shed more light on the influence of DNA replication on the formation and distribution of chromosome aberrations, breakpoints (BP) produced by UV-C and AluI were assigned either to the early replicating short euchromatic arm (Xp(e)) or to the late replicating long heterochromatic arm (Xq(h)) of the Chinese hamster (CHO9) X chromosome. Early (ES) or late (LS) S-phase cells were assessed by pulse incorporating the base analogue 5-bromo-2'-deoxyuridine (BrdU) immediately after UV-C irradiation (30 J/m(2)) or AluI (20 U) poration followed by BrdU immunodetection with FITC-tagged antibodies in metaphase spreads. Short (30 s) UV-C exposures (1 J/m(2)/s) induced BP preferentially in Xq(h) in LS cells and a random distribution of BP along Xp(e) and Xq(h) in ES cells. However, BP induced by long (5 min) UV-C exposures (0.1 J/m(2)/s) clustered according to arm replication time (Xp(e) during ES and Xq(h) along LS). Giemsa-stained metaphases showed elevated frequencies of UV-C induced chromatid-type aberrations and gaps, especially in cells exposed to longer UV-C irradiation. BP induced by AluI clustered in Xp(e) in ES but distributed randomly during LS. In contrast to UV-C, AluI did not produce an increase in the yield of gaps, neither in ES nor in LS cells. Putative mechanisms underlying the observed distributions of chromosome damage in replicating CHO9 cells exposed to UV-C and AluI are discussed. Copyright 2010 S. Karger AG, Basel. PMID: 20407222 [PubMed - indexed for MEDLINE] Publication Types, MeSH Terms, SubstancesPublication Types: Research Support, Non-U.S. Gov't MeSH Terms: Animals CHO Cells Chromosome Breakpoints Cricetinae Cricetulus DNA Damage/drug effects DNA Replication* Euchromatin/metabolism* Heterochromatin/metabolism* Substances: Euchromatin Heterochromatin LinkOut - more resourcesFull Text Sources: S. Karger AG, Basel, Switzerland EBSCO ProQuest Information and Learning Swets Information Services Supplemental Content Related citations Modulation of chromosome damage localization by DNA replication timing. [Int J Radiat Biol. 2006] Modulation of chromosome damage localization by DNA replication timing. Di Tomaso MV, Martínez-López W, Folle GA, Palitti F. Int J Radiat Biol. 2006 Dec; 82(12):877-86. Induction, processing and persistence of radiation-induced chromosomal aberrations involving hamster euchromatin and heterochromatin. [Mutat Res. 2000] Induction, processing and persistence of radiation-induced chromosomal aberrations involving hamster euchromatin and heterochromatin. Puerto S, Marcos R, Ramírez MJ, Creus A, Boei JJ, Meijers M, Natarajan AT, Surrallés J. Mutat Res. 2000 Sep 20; 469(2):169-79. Distribution of breakpoints induced by etoposide and X-rays along the CHO X chromosome. [Cytogenet Genome Res. 2004] Distribution of breakpoints induced by etoposide and X-rays along the CHO X chromosome. Martínez-López W, Folle GA, Cassina G, Méndez-Acuña L, Di-Tomaso MV, Obe G, Palitti F. Cytogenet Genome Res. 2004; 104(1-4):182-7. Review Insights into the mechanisms of sister chromatid exchange formation. [Cytogenet Genome Res. 2004] Review Insights into the mechanisms of sister chromatid exchange formation. Wojcik A, Bruckmann E, Obe G. Cytogenet Genome Res. 2004; 104(1-4):304-9. Review Replication of damaged DNA: molecular defect in xeroderma pigmentosum variant cells. [Mutat Res. 1999] Review Replication of damaged DNA: molecular defect in xeroderma pigmentosum variant cells. Cordonnier AM, Fuchs RP. Mutat Res. 1999 Oct 22; 435(2):111-9. See reviews... See all... Recent activity Clear Turn Off Turn On Asynchronously replicating Eu/heterochromatic regions shape chromosome damage. Asynchronously replicating Eu/heterochromatic regions shape chromosome damage. Cytogenet Genome Res. 2010 ;128(1-3):111-7. Epub 2010 Apr 20 .PubMedpalitti f. (126) PubMedYour browsing activity is empty. Activity recording is turned off. Turn recording back on See more... 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