Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/2204
Title: | CD3 γ/δ in sea bass (Dicentrarchus labrax): molecular characterization and expression analysis | Authors: | Randelli, Elisa Scapigliati, Giuseppe Buonocore, Francesco |
Keywords: | Fish immunology;Aquaculture;Marine biotechnology | Issue Date: | 2011 | Publisher: | Elsevier | Source: | Randelli, E., Scapigliati, G., Buonocore, F. 2011. CD3 γ/δ in sea bass (Dicentrarchus labrax): molecular characterization and expression analysis. "Results in Immunology" 1 (1): 31-35 | Abstract: | The CD3 complex is the common marker on the surface of both ab and gd T cells and is essential for formation of the T-cell receptor complex and for T-cell activation. In this paper, we report the gene cloning and molecular characterization of a CD3g/d homologue in sea bass (Dicentrarchus labrax), the analysis of transcription levels in lymphoid and non-lymphoid organs and the gene regulation after in vitro stimulation with LPS and PHA. Four cysteine residues in the extracellular domain, involved in the constitution of immunoglobulinlike domain, are present in sea bass CD3g/d sequence and they are conserved both in number and position from mammals to teleost sequences. Similar to other known CD3g/ds, in sea bass CD3g/d there is also a conserved immunoreceptor tyrosine-based activation ITAM motif that could be responsible for its individual signal transduction capacity. The real time RT-PCR basal analysis shows the highest level of CD3g/d mRNA in thymus, followed by peripheral blood leucocytes, spleen, gills, gut, liver, head kidney, brain and muscle. The expression analysis under stimuli condition reveals a significant decrease of CD3g/d expression after LPS stimulation and a significant increase after PHA-L stimulation, in agreement with mammals results. In conclusion, these data allow us to affirm that sea bass CD3g/d can be used as a T cell marker and will help in adding new insight on the immune response mechanisms of sea bass. |
Description: | L'articolo è disponibile sul sito dell'editore http://www.elsevier.com |
URI: | http://hdl.handle.net/2067/2204 | ISSN: | 2211-2839 | DOI: | 10.1016/j.rinim.2011.08.003 |
Appears in Collections: | DISA - Archivio della produzione scientifica |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Resultsinimmunol2011.pdf | 787.73 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
10
10
Last Week
0
0
Last month
0
0
checked on Mar 16, 2024
Page view(s)
184
Last Week
0
0
Last month
3
3
checked on Mar 16, 2024
Download(s)
109
checked on Mar 16, 2024
Google ScholarTM
Check
Altmetric
All documents in the "Unitus Open Access" community are published as open access.
All documents in the community "Prodotti della Ricerca" are restricted access unless otherwise indicated for specific documents