Aspetti genetico-molecolari e tecnologici del trasferimento al frumento di geni del Dasypyrum villosum

Abstract

Many breeding programs rely on gene transfer from the wild and cultivated secondary gene-pool (GP-2) for trait enhancement, but so far no reports on transfer of bread-making quality-related genes from the wild GP-2 of wheat have been released. In this paper, we used small- and large-scale tests to analyze wheat introgression lines (ILs) derived from Triticum aestivum cv “Chinese Spring” (CS) Dasypyrum villosum (L.) Candargy (Dv) hybridization. We aimed at ascertaining the efficacy of prolamin subunits encoded by Dv genes for the improvement of wheat end-use grain quality Biochemical and genetic evidences from backcrossing those lines to CS, and the qualitative results obtained from the lines grown for two years at two localities demonstrated that the ascertained positive effects of the Dv genes on wheat-flour end-use quality correlate weakly with the size of the introgressed Dv chromatin that contained those genes or the protein content of the flour. Dv chromatin including genes at the Glu-V1 locus, coding for high molecular weight glutenins in Dv, significantly improves wheat bread-making quality, and chromatin from the short arm of 6V chromosome improves also protein and micronutrient (Fe and Zn) content.