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Title: | Intestinal T cells of Dicentrarchus labrax (L.): gene expression and functional studies | Authors: | Picchietti, Simona Guerra, Laura Bertoni, Fabrizio Randelli, Elisa Belardinelli, Maria Cristina Buonocore, Francesco Rombout, Jan Scapigliati, Giuseppe Abelli, Luigi |
Keywords: | Fish Immunology;Evolution of immune system;GALT;Thymus;RNA in situ hybridization;Real-time PCR;Cytotoxic T cells;Dicentrarchus labrax | Issue Date: | 2011 | Publisher: | Elsevier | Source: | Picchietti, S. et al. 2011. Intestinal T cells of Dicentrarchus labrax (L.): gene expression and functional studies. "Fish & Shellfish Immunology" 30 (2): 609-617 | Abstract: | Cellular and molecular data have evidenced a gut-associated lymphoid tissue in a variety of teleost species, abundantly containing T cells, whose origin, selection and functions are still unclear. This study reports CD4, CD8-α, MHCI-α, MHCII-β, rag-1 and TCR-β gene transcription along the intestine (anterior, middle and posterior segments) and in the thymus of one year-old Dicentrarchus labrax (L.). Real-time PCR findings depicted a main role of the thymus in T cell development, but also rag-1 and CD8-α transcripts are detected in the intestine, having significant expression in the posterior segment. In the whole intestine TCR-β and CD8-α exceeded CD4 transcripts. RNA ISH confirmed these data and detailed that mucosal CD8-α+ cells were especially numerous in the epithelium and in aggregates in the lamina propria. Regional differences in T cell-specific gene expressions are first described in the intestine of a bony fish. High non-specific cytotoxic activity against xenogeneic and allogeneic cells was found in lymphocytes purified from the intestinal mucosa, providing further insight into their local defence roles. |
Description: | L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com/ |
URI: | http://hdl.handle.net/2067/2183 | ISSN: | 1050-4648 | DOI: | 10.1016/j.fsi.2010.12.006 |
Appears in Collections: | DISA - Archivio della produzione scientifica |
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IntTcells.doc | 30 kB | Microsoft Word | View/Open |
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