Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/2180
Title: | Analysis of the expression and modulation of selected immune-related gene transcripts in the DLEC cell line from European sea bass (Dicentrarchus labrax) | Authors: | Buonocore, Francesco Randelli, Elisa Lorenzen, Niels Einer-Jensen, Katja Scapigliati, Giuseppe |
Keywords: | Fish Immunology;Evolution of the immune system | Issue Date: | 2011 | Publisher: | OMICS Publishing Group | Source: | Buonocore, F. et al. 2011. Analysis of the expression and modulation of selected immune-related gene transcripts in the DLEC cell line from European sea bass (Dicentrarchus labrax) "Journal of Aquaculture Research & Development" 2 (1): 105 | Abstract: | Cell lines have been established from different fish species especially for virus isolation and for studying cell-pathogen interactions, and therefore are of interest in aquaculture. In this paper, we have investigated the presence and the regulation of some immune genes in the DLEC (Dicentrarchus labrax embryonic cells) cell line from European sea bass (Dicentrarchus labrax L.) to preliminary elucidate their action. The basal expression of the selected genes (interleukin- 1β (IL-1β), cyclooxygenase-2 (COX-2), transforming growth factor-β (TGF-β), CD8-α, major histocompatibility complex II-β (MHC II-β), interferon (IFN) and Mx protein (Mx)) have been investigated and, successively, their modulation have been studied both after stimulation with different mitogen agents and after a transfection with a sequence codifying for the coat protein of a fish nervous necrosis virus (NNV). The results have evidenced that the inflammatory molecules (IL-1β, COX-2, TGF-β), constitutively expressed by the DLEC cell line, are not up-regulated by the stimulation with lipopolysaccharide (LPS) from E. coli, whether the expression of the T-cell marker transcripts (CD8-α, MHC II-β) is influenced by the action of a lectin from Phaseolus vulgaris (PHA-L). Finally, the expression of the coat NNV protein in the DLEC cell line, after the transfection, led to an high up-regulation of IFN and Mx gene transcripts. These data suggest that the DLEC cell line recognize specific pathogen-associated molecular patterns (PAMPs) and, therefore, could be useful for studying T-cell pathways and viral responses in sea bass avoiding the use of live test animals. |
URI: | http://hdl.handle.net/2067/2180 | ISSN: | 2155-9546 | DOI: | 10.4172/2155-9546.1000105 |
Appears in Collections: | DISA - Archivio della produzione scientifica |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
DLECopenacccessproof.pdf | 2.01 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
20
2
Last Week
0
0
Last month
0
0
checked on Mar 15, 2024
Page view(s)
189
Last Week
0
0
Last month
7
7
checked on Mar 16, 2024
Download(s)
82
checked on Mar 16, 2024
Google ScholarTM
Check
Altmetric
All documents in the "Unitus Open Access" community are published as open access.
All documents in the community "Prodotti della Ricerca" are restricted access unless otherwise indicated for specific documents