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http://hdl.handle.net/2067/2069
Title: | Zinc deficiency suppresses the development of oral tolerance in rats | Authors: | Finamore, Alberto Roselli, Marianna Merendino, Nicolò Nobili, Fabio Vignolini, Francesco Mengheri, Elena |
Keywords: | Zinc deficiency;Oral tolerance;Intestine;Cytokines;Rats | Issue Date: | 2003 | Publisher: | American Society for Nutritional Sciences | Source: | Finamore, A. et al. 2003. Zinc deficiency suppresses the development of oral tolerance in rats. "Journal of nutrition" 133 (1): 191-198 | Abstract: | Oral tolerance is a specific immune unresponsiveness to food antigens to prevent hypersensitivity reactions. We investigated whether zinc deficiency affects oral tolerance. Rats were fed a control (C) or zincdeficient (ZD) diet, or pair-fed (PF) to ZD rats for 28 d. Beginning on d 7, rats were administered ovalbumin (OVA) orally to induce tolerance, or PBS 3 times/wk, and were then immunized by OVA injection. The proliferation of mesenteric lymph node (MLN) and spleen lymphocytes after in vitro OVA stimulation and the delayed-type hypersensitivity were higher in OVA-fed ZD than in OVA-fed C rats and not different between OVA- and PBS-fed ZD rats, indicating a suppression of tolerance. Lymphocyte proliferation did not differ between PF and C rats. Expressions of cytokines involved in oral tolerance, i.e., interleukin (IL)-4, IL-10 and transforming growth factor- , were higher in OVA- than in PBS-fed C rats, but not in ZD rats. Apoptosis was higher in OVA- than in PBS-fed C rats but not different between OVA- and PBS-fed ZD rats. Inflammation and ulcerations that were not present in ZD rats on d 7 (ZD7 ) developed in OVA- or PBS-fed ZD rats. Compared with ZD7 rats, tumor necrosis factor- and cytokine-induced neutrophil chemoattractant were higher in OVA- and PBS-fed ZD rats, whereas interferon- increased only in OVA-fed ZD rats. In conclusion, zinc deficiency suppresses oral tolerance through dysregulation of cytokine expression and lack of antigen-specific clonal deletion. We suggest that abrogation of tolerance may lead to development of mucosal inflammation and damage. |
Description: | L'articolo è disponibile sul sito dell'editore http://jn.nutrition.org |
URI: | http://hdl.handle.net/2067/2069 | ISSN: | 0022-3166 |
Appears in Collections: | DECOS - Archivio della produzione scientifica |
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