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Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1933

Title: Mutation scanning and genotyping by high-resolution DNA melting analysis in olive germplasm
Authors: Muleo, Rosario
Colao, Maria Chiara
Miano, Dario
Cirilli, Marco
Intrieri, Maria Carmela
Baldoni, Luciana
Rugini, Eddo
Keywords: HRM
High-resolution melting
Genotyping
Melting curve analysis
SNP
ARMS-PCR
Issue Date: 2009
Publisher: NRC Research Press
Citation: Muleo, R. et al. 2009. Mutation scanning and genotyping by high-resolution DNA melting analysis in olive germplasm. "Genome" 52(3): 252-260
Abstract: The application of high-resolution melting (HRM) analysis of DNA is reported l'or scanning and genotyping Olea europaea germplasm. To test the sensitivity of the method, a functional gene marker, phytochrome A (phyA), was used, since this gene is correlated with important traits for the ecology of the species. We have designed a set of oligos able to produce amplicons of 307 bp to scan for the presence of single polymorphic mutations in a specific phyA fragment encompassing the chromophore altac
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Description: L'articolo é disponibile sul sito dell'editore: http://www.nrcresearchpress.com
DOI: 10.1139/G09-002
URI: http://hdl.handle.net/2067/1933
ISSN: 0831-2796
Appears in Collections:DIPROV - Archivio della produzione scientifica

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