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http://hdl.handle.net/2067/1790
Title: | A novel and efficient oxidative functionalization of lignin by layer-by-layer immobilised Horseradish peroxidase | Authors: | Perazzini, Raffaella Saladino, Raffaele Guazzaroni, Melissa Crestini, Claudia |
Keywords: | 31PNMR;Enzyme immobilisation;Horseradish peroxidase;Layer by layer;Lignin;Lignin oxidation | Issue Date: | 2011 | Publisher: | Elsevier Ltd. | Source: | Perazzini R. et al. 2011. A novel and efficient oxidative functionalization of lignin by layer-by-layer immobilised Horseradish peroxidase. "Bioorganic and Medicinal Chemistry" 19(1): 440-447 | Abstract: | Horseradish peroxidase (HRP) was chemically immobilised onto alumina particles and coated by polyelectrolytes layers, using the layer-by-layer technique. The reactivity of the immobilised enzyme was studied in the oxidative functionalisation of softwood milled wood and residual kraft lignins and found higher than the free enzyme. In order to investigate the chemical modifications in the lignin structure, quantitative 31P NMR was used. The immobilised HRP showed a higher reactivity with respect to the native enzyme yielding extensive depolymerisation of lignin. |
Description: | L'articolo è disponibile sul sito dell'editore: http://www.sciencedirect.com |
URI: | http://hdl.handle.net/2067/1790 | ISSN: | 09680896 |
Appears in Collections: | DABAC - Archivio della produzione scientifica |
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bioorgMedChem2010Cover.pdf | 183.11 kB | Adobe PDF | View/Open |
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