Micro-methods for genotypic screening of hazelnut (Corylus avellana L.) seeds to accelerate breeding for seed metabolite improvement

Abstract

We used hazelnut full-sib (FS) seed progenies from controlled pollination for efficiency testing of a multi-micro-method procedure comprising: a generalized low reagent-cost and low amount of extracting-tissue protocol for DNA purification and SSR seed genotyping, nuclear magnetic resonance (NMR) detection of analyte spectra for phenotyping metabolite content, and in vitro culture for clonal propagation of the embryo genotype.These micro-methods can be integrated into breeding programs for hazelnut fruit metabolite improvement.