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Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1765

Title: A generalized procedure to prepare PCR-grade DNA from different Corylus avellana L. plant tissues for parentage analysis.
Authors: Kuzmanović, Ljiljana
Rugini, Eddo
De Pace, Ciro
Keywords: DNA purification
Corylus avellana
Hazelnut
PCR
RAPD
SSR
Genotyping
Parentage analysis
Issue Date: 2009
Publisher: ISHS
Citation: Kuzmanović L., Rugini E., De Pace C. 2009. A generalized procedure to prepare pcr-grade dna from different corylus avellana l. Plant tissues for parentage analysis. "Acta Horticulturae" 814: 639-644
Abstract: A generalized protocol has been developed for an easy DNA purification from different hazelnut tissues. The extraction method avoids the use of liquid nitrogen, requires minimal amount (50 mg) of plant tissue, and allows the recovery of 0.8-1.3 μg high molecular weight and PCR-grade DNA from cotyledons and leaves and 0.2-0.3 μg of DNA from the pollen. The purified DNA has the quality to PCR-amplify and is neatly visualized in agarose gels. This protocol could improve and speed-up not only parent
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Description: L'articolo è disponibile sul sito dell'editore: http://www.ishs.org
URI: http://hdl.handle.net/2067/1765
ISBN: 978-90-66052-90-1
Appears in Collections:DABAC - Archivio della produzione scientifica
DIPROV - Archivio della produzione scientifica

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