Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/1725
Title: | Development of Innovative Molecular Methods for the Detection and the Identification of Pseudomonas spp. in Environmental and Clinical Samples | Authors: | Calisti, Cecilia Ruzzi, Maurizio |
Keywords: | Pseudomonas species;T-RFLP;Molecular typing;PCR;T-RFLP;16S ribosomal DNA;gyrB gene | Issue Date: | 2009 | Publisher: | UASVM Animal Science and Biotechnologies | Source: | Calisti C., Ruzzi, M. 2009. Development of Innovative Molecular Methods for the Detection and the Identification of Pseudomonas spp. in Environmental and Clinical Samples. "Bulletin UASVM Animal Science and Biotechnologies" 66 (1-2) | Abstract: | We have developed a rapid, reliable and sensitive method to analyze, detect and trace dissemination of pathogenic and spoilage Pseudomonas species in foods and environments. The molecular identification of Pseudomonas species was achieved using a PCR-based assay with primer sets specific for 16S ribosomal DNA and gyrB genes. This PCR assay was found to provide highly genus-specific detection and could be successfully used to identify Pseudomonas in microbial consortia where these bacteria were not abundant. By coupling the PCR assay for 16S rDNA gene to a T-RFLP technique, identification of Pseudomonas strains at species level could be obtained without cultivation. PCR with a combination of two target sequences (multiplex PCR) appeared to be the optimum choice for discriminating between Pseudomonas and closely related genera. |
URI: | http://hdl.handle.net/2067/1725 | ISSN: | 1843-5262 |
Appears in Collections: | DABAC - Archivio della produzione scientifica |
Show full item record
Page view(s)
83
Last Week
0
0
Last month
1
1
checked on Mar 16, 2024
Download(s)
85
checked on Mar 16, 2024
Google ScholarTM
Check
All documents in the "Unitus Open Access" community are published as open access.
All documents in the community "Prodotti della Ricerca" are restricted access unless otherwise indicated for specific documents