Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/1688
Title: | Molecular and structural characterisation of a macrophage migration inhibitory factor from sea bass (Dicentrarchus labrax L.) | Authors: | Buonocore, Francesco Randelli, Elisa Facchiano, Angelo M. Pallavicini, Alberto Modonut, Martina Scapigliati, Giuseppe |
Keywords: | Fish Immunology;Molecular Biology and Evolution;3D structure;European sea bass;Dicentrarchus labrax;MIF;Real-time PCR | Issue Date: | 2010 | Publisher: | Elsevier | Source: | Buonocore, F. et al. 2010. Molecular and structural characterisation of a macrophage migration inhibitory factor from sea bass (Dicentrarchus labrax L.). "Veterinary Immunology and Immunopathology" 136(3-4): 297-304 | Abstract: | The macrophage migration inhibitory factor (MIF) is a cytokine produced in numerous cell types, mainly T lymphocytes and macrophages, in response to inflammatory stimuli. In this paper we report the identification of a cDNA encoding a MIF molecule from sea bass (Dicentrarchus labrax L.), its expression analysis and its 3D structure obtained by template-based modelling. The sea bass MIF cDNA consists of 609 bp that translates in one reading frame to give the entire molecule containing 115 amino acids. The sequence contains three cysteine residues in conserved positions compared to human MIF and most Teleost fishes, with the exception of zebrafish and carp. The Cys57-Ala58-Leu59-Cys60 motif, present inside the stretch important for JAB1-interaction and mediator of the thiol-protein oxidoreductase activity of MIF, is conserved in sea bass, together with the Pro2 residue that is crucial for the tautomerase catalytic activity. Real time PCR analyses revealed that MIF is constitutively expressed in all selected tissues and organs, with the highest mRNA level observed in thymus. MIF expression was induced after 4 hours in vitro stimulation of head kidney leukocytes with LPS and decreased after 24 hours. The predicted 3D model of sea bass MIF has been used to verify the presence of structural requirements for its known biological activities. |
Description: | L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com/ |
URI: | http://hdl.handle.net/2067/1688 | ISSN: | 0165-2427 | DOI: | 10.1016/j.vetimm.2010.03.007 |
Appears in Collections: | DISA - Archivio della produzione scientifica |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
MIFpaper.doc | 27 kB | Microsoft Word | View/Open |
SCOPUSTM
Citations
10
17
Last Week
0
0
Last month
1
1
checked on Sep 18, 2023
Page view(s)
192
Last Week
0
0
Last month
3
3
checked on Mar 16, 2024
Download(s)
59
checked on Mar 16, 2024
Google ScholarTM
Check
Altmetric
All documents in the "Unitus Open Access" community are published as open access.
All documents in the community "Prodotti della Ricerca" are restricted access unless otherwise indicated for specific documents