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Title: | Effect of storage conditions of blood on radiation-induced chromosomal aberrations and apoptosis in human lymphocytes |
Authors: | Belloni, Paola Pepe, Gaetano Palitti, Fabrizio |
Keywords: | Apotosis Blood storage Chromosomal aberrations X-rays |
Issue Date: | 2010 |
Publisher: | Elsevier |
Citation: | Belloni P. Pepe G. Palitti F. 2010. Effect of storage conditions of blood on radiation-induced chromosomal aberrations and apoptosis in human lymphocytes. "Mutation Research" 695(1-2): 35-39 |
Abstract: | To evaluate the effect of storage conditions of blood on the direct relationship between radiation-induced
chromosome aberrations and apoptosis in human peripheral blood lymphocytes,whole bloodwas irradi-
atedwith 3Gy X-rays. Directly after irradiation, a sample of bloodwas analyzed for chromosome damage
and proliferation index, after phytohaemagglutinin stimulation and incubation at 37 ◦
C for 56 h. Blood
samples were stored for 48 h at 4 and 20 ◦
C with or without phytohaemagglutinin and analyzed for
cell viability and apoptosis at 0, 24 and 48 h storage time. After 48 h of storage, unstimulated cultures
were stimulated to proliferate. These samples and cultures stimulated immediately before storage were
incubated at 37 ◦
C for 56 h and analyzed for chromosome damage and proliferation index. Metaphases
were examined for the presence of dicentrics, excess acentrics, and rings. Storage at 20 ◦
Cwithout phyto-
haemagglutinin for 48 h increases significantly the yield of apoptosis and decreases significantly the yield
of dicentrics. During 48 h of storage time the presence of phytohaemagglutinin and the temperature of
4 ◦
C protected the irradiated lymphocytes from apoptosis allowing accurate estimation of the real yield
of radiation-induced chromosome damage. Therefore these blood-storage conditions enable analysis in
metaphase and may offer some advantages for biodosimetry of absorbed radiation dose. ...more |
Description: | L'articolo è disponibile sul sito dell'editore: http://www.sciencedirect.com |
URI: | http://hdl.handle.net/2067/1674 |
ISSN: | ISSN: 0027-5107 |
Appears in Collections: | DABAC - Archivio della produzione scientifica
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