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Title: | Effect of storage conditions of blood on radiation-induced chromosomal aberrations and apoptosis in human lymphocytes | Authors: | Belloni, Paola Pepe, Gaetano Palitti, Fabrizio |
Keywords: | Apotosis;Blood storage;Chromosomal aberrations;X-rays | Issue Date: | 2010 | Publisher: | Elsevier | Source: | Belloni P. Pepe G. Palitti F. 2010. Effect of storage conditions of blood on radiation-induced chromosomal aberrations and apoptosis in human lymphocytes. "Mutation Research" 695(1-2): 35-39 | Abstract: | To evaluate the effect of storage conditions of blood on the direct relationship between radiation-induced chromosome aberrations and apoptosis in human peripheral blood lymphocytes,whole bloodwas irradi- atedwith 3Gy X-rays. Directly after irradiation, a sample of bloodwas analyzed for chromosome damage and proliferation index, after phytohaemagglutinin stimulation and incubation at 37 ◦ C for 56 h. Blood samples were stored for 48 h at 4 and 20 ◦ C with or without phytohaemagglutinin and analyzed for cell viability and apoptosis at 0, 24 and 48 h storage time. After 48 h of storage, unstimulated cultures were stimulated to proliferate. These samples and cultures stimulated immediately before storage were incubated at 37 ◦ C for 56 h and analyzed for chromosome damage and proliferation index. Metaphases were examined for the presence of dicentrics, excess acentrics, and rings. Storage at 20 ◦ Cwithout phyto- haemagglutinin for 48 h increases significantly the yield of apoptosis and decreases significantly the yield of dicentrics. During 48 h of storage time the presence of phytohaemagglutinin and the temperature of 4 ◦ C protected the irradiated lymphocytes from apoptosis allowing accurate estimation of the real yield of radiation-induced chromosome damage. Therefore these blood-storage conditions enable analysis in metaphase and may offer some advantages for biodosimetry of absorbed radiation dose. |
Description: | L'articolo è disponibile sul sito dell'editore: http://www.sciencedirect.com |
URI: | http://hdl.handle.net/2067/1674 | ISSN: | ISSN: 0027-5107 |
Appears in Collections: | DABAC - Archivio della produzione scientifica |
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