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Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1610

Title: Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
Authors: Paolacci, Anna Rita
Tanzarella, Oronzo A.
Porceddu, Enrico
Ciaffi, Mario
Keywords: Quantitative RT-PCR
Reference genes
Wheat
RT-PCR quantitativa
Geni di riferimento
Frumento
Issue Date: 2009
Publisher: BioMed Central
Citation: Paolacci A.R. et al. 2009. Identification and validation of reference genes for quantitative RT-PCR normalization in wheat "BMC Molecular Biology" 10 (11): 1-27
Abstract: Background: Usually the reference genes used in gene expression analysis have been chosen for their known or suspected housekeeping roles, however the variation observed in most of them hinders their effective use. The assessed lack of validated reference genes emphasizes the importance of a systematic study for their identification. For selecting candidate reference genes we have developed a simple in silico method based on the data publicly available in the wheat databases Unigene and TIG
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Description: L'articolo è disponibile sul sito dell'editore: http://www.biomedcentral.com
DOI: 10.1186/1471-2199-10-11
URI: http://hdl.handle.net/2067/1610
ISSN: 1471-2199
Appears in Collections:DABAC - Archivio della produzione scientifica

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