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Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1563

Title: Lymphocyte differentiation in sea bass thymus: CD4 and CD8-α gene expression studies
Authors: Picchietti, Simona
Guerra, Laura
Buonocore, Francesco
Randelli, Elisa
Fausto, Anna Maria
Abelli, Luigi
Keywords: Dicentrarchus labrax
Thymus
CD4
In situ hybridization
Development
Positive selection
Thymocytes
Issue Date: 2009
Publisher: Elsevier
Citation: Picchietti, S. et al. 2009. Lymphocyte differentiation in sea bass thymus: CD4 and CD8-α gene expression studies. "Fish and Shellfish Immunology" 27(1): 50-56
Abstract: Different developmental stages (from eggs to 1-year-old juveniles) of the teleost fish Dicentrarchus labrax (L.) were assayed for CD4 gene expression. RT-PCR revealed the appearance of CD4 transcripts in postlarvae from 51 days post-hatching (dph). This finding overlaps the first detection of CD8-a mRNA. Real-time PCR with specific primers quantified CD4, CD8-a and TCR-b transcripts in larvae and postlarvae (25, 51, 75 and 92 dph) and 1-year-old thymus. At 92 dph, TcR-b and CD8-a transcripts
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Description: L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com/
DOI: 10.1016/j.fsi.2009.04.003
URI: http://hdl.handle.net/2067/1563
ISSN: 1050-4648
Appears in Collections:DISA - Archivio della produzione scientifica

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