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Title: | The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus) | Authors: | Ferguson, R.M.W. Merrifield, Daniel L. Harper, Glenn M. Rawling, Mark D. Mustafa, Sanaa Picchietti, Simona Balcàzar, Josè Luis Davies, Simon J. |
Keywords: | Fish;Health;Histology;PCR-DGGE;Probiotic | Issue Date: | 2010 | Publisher: | Wiley-Blackwell | Source: | Ferguson, R.M.W. et al. 2010. The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus). "Journal of Applied Microbiology" 109(3): 851-862 | Abstract: | Aim: To assess Pediococcus acidilactici as a dietary supplement for on-growing red tilapia (Oreochromis niloticus). Methods and Results: Tilapia were fed either a control diet or control diet supplemented with Ped. acidilactici at 107 CFU g)1 for 32 days. Ped. acidilactici colonized the intestinal tract and significantly affected the intestinal microbial communities. PCR-DGGE revealed direct antagonism of gastric Ped. acidilactici with an endogenous uncultured bacterium during a period of reverting to nonsupplemented feeding. Light microscopy revealed that gut integrity and leucocyte levels were unaffected by Ped. acidilactici; however, blood leucocyte levels and serum lysozyme activity were elevated after 14-days’ feeding. No significant improvements in growth performance were observed at the end of the trial (day 32), but survival was significantly higher in the probiotic group. Conclusions: The study demonstrates that oral supplementation of Ped. acidilactici modulates intestinal bacterial communities in on-growing red tilapia and also stimulates some aspects of the nonspecific immune response. Significance and Impact of the study: To our knowledge this is the first study assessing the effects of probiotics on the gut microbiota of tilapia using culture-independent methods. Such methods are crucial to understand the mechanisms which underpin and mediate host benefits. |
Description: | L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com/ |
URI: | http://hdl.handle.net/2067/1554 | ISSN: | 1364-5072 | DOI: | 10.1111/j.1365-2672.2010.04713.x |
Appears in Collections: | DISA - Archivio della produzione scientifica |
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JAM 2010.pdf | 70.67 kB | Adobe PDF | View/Open |
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