Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1459
Title: Cytotoxic effects and apoptotic signalling mechanisms of the sesquiterpenoid euplotin C, a secondary metabolite of the marine ciliate Euplotes crassus, in tumour cells
Authors: Cervia, Davide
Martini, Davide
Garcia-Gil, Mercedes
Di Giuseppe, Graziano
Guella, Graziano
Dini, Fernando
Bagnoli, Paola
Keywords: Ca2+ stores;Caspases;Cell death;Cytochrome c;Endoplasmic reticulum
Issue Date: 2006
Publisher: Springer Verlag
Source: Cervia, D. et al. 2006. Cytotoxic effects and apoptotic signalling mechanisms of the sesquiterpenoid euplotin C, a secondary metabolite of the marine ciliate Euplotes crassus, in tumour cells. "Apoptosis" 11:829-843
Abstract: 
Most antitumour agents with cytotoxic properties induce apoptosis. The lipophilic compound euplotin C, isolated from the ciliate Euplotes crassus, is toxic to a number of different opportunistic or pathogenic microorganisms, although its mechanism of action is currently unknown. We report here that euplotin C is a powerful cytotoxic and pro-apoptotic agent in mouse AtT-20 and rat PC12 tumour-derived cell lines. In addition, we provide evidence that euplotin C treatment results in rapid activation of ryanodine receptors, depletion of Ca2+ stores in the endoplasmic reticulum (ER), the release of cytochrome c from the mitochondria, activation of caspase-12, and activation of caspase-3, leading to apoptosis. Intracellular Ca2+ overload is an early event which induces apoptosis and is parallelled by ER stress and the release of cytochrome c, whereas caspase-12 may be activated by euplotin C at a later stage in the apoptosis pathway. These events, either independently or concomitantly, lead to the activation of the caspase-3 and its downstream effectors, triggering the cell to undergo apoptosis. These results demonstrate that euplotin C may be considered for the design of cytotoxic and pro-apoptotic new drugs.
Description: 
L'articolo è disponibile sul sito dell'editore http://www.springerlink.com/
URI: http://hdl.handle.net/2067/1459
ISSN: 1360-8185
DOI: 10.1007/s10495-006-5700-3
Appears in Collections:DISA - Archivio della produzione scientifica

Files in This Item:
File Description SizeFormat
Cervia et al-Apoptosis 2006 1.pdf157.46 kBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations 5

38
Last Week
0
Last month
1
checked on Mar 16, 2024

Page view(s)

76
Last Week
0
Last month
0
checked on Mar 16, 2024

Download(s)

170
checked on Mar 16, 2024

Google ScholarTM

Check

Altmetric


All documents in the "Unitus Open Access" community are published as open access.
All documents in the community "Prodotti della Ricerca" are restricted access unless otherwise indicated for specific documents