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Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1450

Title: Comparison of functional profiles at human recombinant somatostatin sst2 receptor: simultaneous determination of intracellular Ca2+ and luciferase expression in CHO-K1 cells
Authors: Nunn, Caroline
Cervia, Davide
Langenegger, Daniel
Tenaillon, Laurent
Bouhelal, Rochdi
Hoyer, Daniel
Keywords: Somatostatin receptor subtype 2
CHO-K1 cells
FLIPR II
SRE-luciferase
Duplex assay
Pertussis toxin
Thapsigargin
Issue Date: 2004
Publisher: Wiley-Blackwell
Citation: Nunn, C. et al. 2004. Comparison of functional profiles at human recombinant somatostatin sst2 receptor: simultaneous determination of intracellular Ca2+ and luciferase expression in CHO-K1 cells. "British Journal of Pharmacology" 142(1):150-160
Abstract: 1. Somatostatin (somatotropin release inhibiting factor; SRIF) acts via five G protein coupled receptors (sst1-sst5) which modulate multiple cellular effectors. The aim of this study was to compare two functional effects of the human sst2 receptor stably expressed in CHO-K1 cells in a single experiment using a duplex assay for intracellular calcium and serum response element (SRE)-driven luciferase expression. 2. Intracellular calcium was measured using a FLuorometric Imaging Plate Reader II
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Description: L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com/
DOI: 10.1038/sj.bjp.0705735
URI: http://hdl.handle.net/2067/1450
ISSN: 0007-1188
Appears in Collections:DISA - Archivio della produzione scientifica

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