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http://hdl.handle.net/2067/1430
Title: | Somatostatin-induced control of cytosolic free calcium in pituitary tumour cells | Authors: | Petrucci, Cristina Cervia, Davide Buzzi, Marco Biondi, Carla Bagnoli, Paola |
Keywords: | Sst2 receptor;Agonists;Antagonist;Intracellular Ca2+;Cyclic AMP-dependent pathways;Cell culture;Fluorimetry;Confocal immunofluorescence | Issue Date: | 2000 | Publisher: | Nature Publishing Group | Source: | Petrucci, C. et al. 2000. Somatostatin-induced control of cytosolic free calcium in pituitary tumour cells. "British Journal of Pharmacology" 129(3): 471-484 | Abstract: | 1 In rat pituitary tumor cells (GC cells), spontaneous oscillations of the intracellular concentration of Ca2+ ([Ca2+]i) induce growth hormone (GH) secretion that is inhibited by octreotide, a somatostatin (SRIF) agonist which binds to SRIF subtype (sst) receptor 2. The effects of its functional activation on the control of [Ca2+]i were investigated using fluorimetric measurements of [Ca2+]i. 2 SRIF decreases the basal [Ca2+]i and the [Ca2+]i rise in response to forskolin (FSK) through the inhibition of L-type voltage-dependent Ca2+ channels. 3 Pretreatment with octreotide or with L-Tyr8Cyanamid 154806, a sst2 receptor antagonist, abolishes the SRIF-induced inhibition of [Ca2+]i. Octreotide is known to operate through agonist-induced desensitization, while the antagonist operates through receptor blockade. 4 sst1 and sst2 receptor-immunoreactivities (-IRs) are localized to cell membranes. sst2, but not sst1 receptor-IR, internalizes after cell exposure to octreotide. 5 SRIF-induced inhibition of basal [Ca2+]i or FSK-induced Ca2+ entry is blocked by pertussis toxin (PTX). 6 FSK-induced cAMP accumulation is only partially decreased by SRIF or octreotide, indicating that sst2 receptors are coupled to intracellular pathways other than adenylyl cyclase (AC) inhibition. 7 In the presence of H-89, an inhibitor of cAMP-dependent protein kinase (PKA), SRIF-induced inhibition of basal [Ca2+]i is still present, although reduced in amplitude. 8 SRIF inhibits [Ca2+]i by activating sst2 receptors. Inhibition of AC activity is only partly responsible for this effect, and other transduction pathways may be involved. |
Description: | L'articolo è disponibile sul sito del nuovo editore http://onlinelibrary.wiley.com/ |
URI: | http://hdl.handle.net/2067/1430 | ISSN: | 0007-1188 | DOI: | 10.1038/sj.bjp.0703075 |
Appears in Collections: | DISA - Archivio della produzione scientifica |
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Petrucci et al BJP 2000 1.pdf | 75.89 kB | Adobe PDF | View/Open |
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