Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/142
Title: | Lymphocyte functions in overconditioned cows around parturition | Authors: | Lacetera, Nicola Scalia, Daniela Bernabucci, Umberto Ronchi, Bruno Pirazzi, Daniela Nardone, Alessandro |
Keywords: | Periparturient cow;Body condition score;Lymphocyte function | Issue Date: | 2005 | Publisher: | American Dairy Science Association | Source: | Lacetera, N.et al. 2005. Lymphocyte functions in overconditioned cows around parturition. "Journal of Dairy Science" 88: 2010-2016 | Abstract: | The objective of the study was to evaluate the relationships between body condition and lymphocyte functions in periparturient dairy cows. Thirty days before expected calving, 21 Holstein cows were categorized as thin (n = 6), medium (n = 8), or overconditioned (n = 7) based on body condition score (BCS). Blood samples were collected on 21, 14, 7, and 3 d before calving and on d 3, 7, 14, 21, 28, and 35 after parturition. An aliquot of blood was used to determine plasma nonesterified fatty acids (NEFA) and glucose. At 14 and 7 d before, and 14 and 35 d after calving, a second aliquot of blood was used to assess peripheral blood mononuclear cell (PBMC) functions: DNAsynthesis, immunoglobulin (Ig) M, and interferon-gamma (IFN-γ) secretion after mitogen stimulation. During the experiment, all 21 cows showed a decline in BCS. Overconditioned cows lost significantly more BCS than thin cows. After calving, overconditioned cows had higher plasma NEFA compared with thin and medium cows. Conversely, plasma glucose never differed between the 3 categories of cows. Regardless of BCS, DNA synthesis and IgM secretions were significantly lower in PBMC isolated on 7 d before calving compared with those recorded 14 and 35 d after parturition. Conversely, PBMCfrom the 21 cows did not show any change of IFN-γ secretion during the experimental period. Taking into consideration the BCS categories, PBMC isolated from overconditioned cows presented lower IgM secretion compared with thin cows on d 14 and 35 after calving. Furthermore, PBMC isolated from overconditioned cows secreted less IFN-γ compared with thin and medium cows on d 7 before calving. The DNA synthesis of PBMC stimulated with the 3 mitogens did not differ between the 3 categories of cows. In conclusion, immunodepression occurring in cows around calving would be particularly evident in overconditioned cows. |
URI: | http://hdl.handle.net/2067/142 | ISSN: | 0022-0302 (print) 1525-3198 (online) |
Appears in Collections: | DiPA - Archivio della produzione scientifica |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
05_lacetera_JDS_201_2016.pdf | 1.49 MB | Adobe PDF | View/Open |
Page view(s)
101
Last Week
0
0
Last month
1
1
checked on Mar 16, 2024
Download(s)
128
checked on Mar 16, 2024
Google ScholarTM
Check
This item is licensed under a Creative Commons License