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Title: | Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion | Authors: | Lionetti, Vincenzo Francocci, Fedra Ferrari, Simone Volpi, Chiara Bellincampi, Daniela Galletti, Roberta D'Ovidio, Renato De Lorenzo, Giulia Cervone, Felice |
Keywords: | Biofuel;Pectin;Plant cell wall;Pectin methyl esterase inhibitor;Polygalacturonase;Biocarburante;Pectina;Parete cellulare vegetale;Inibitore della pectin metil esterasi;Poligalatturonasi | Issue Date: | 2010 | Publisher: | National Academy of Sciences | Source: | Lionetti, V. et al. 2010. Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion. "PNAS" 107(2): 616-621. | Abstract: | Plant cell walls represent an abundant, renewable source of biofuels and other useful products. The major bottleneck for the industrial scale-up of their conversion to simple sugars (saccharification) is their recalcitrance to enzymatic hydrolysis. We demonstrated that the structure of pectin affects the exposure of cellulose to enzymes and consequently the process of saccharification. Le pareti cellulari vegetali rappresentano una abbondante e rinnovabile sorgente di biocarburanti ed altri prodotti utili. Una delle maggiori difficoltà nell’utilizzo delle pareti cellulari a livello industriale è la loro conversione in zuccheri semplici (saccarificazione) in quanto sono estremamente resistenti all’idrolisi enzimatica. In questo lavoro dimostriamo che la struttura della pectina influenza l’esposizione della cellulosa all’azione enzimatica e conseguentemente il processo di saccarificazione. |
URI: | http://hdl.handle.net/2067/1356 | ISSN: | 0027-8424 | DOI: | 10.1073/pnas.0907549107 |
Appears in Collections: | DABAC - Archivio della produzione scientifica |
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Lionetti et al.pdf | 252.85 kB | Adobe PDF | View/Open |
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