http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Sat, 25 May 2013 18:27:57 GMT 2013-05-25T18:27:57Z http://hdl.handle.net/2067/1893 Title: Transcriptomic analysis of two sheep breeds during lactation, using a new custom microarray platform Authors: Bongiorni, Silvia; Chillemi, Giovanni; Prosperini, Gianluca; Bueno, Susana; Signorelli, Federica; Moioli, Bianca; Pariset, Lorraine Abstract: We aim at understanding the genomic influence on milk quality and synthesis by comparing two sheep breeds using sheep-specific microarray technology. From sheep ESTs deposited at NCBI we generated a chip carrying about 22,000 non-redundant features in quadruplicate, achieving very good technical outcomes. Oligos were in situ generated on chip using the Combimatrix equipment. We analysed the mammary transcriptome in individuals of two sheep breeds at two lactation stages, to identify genes controlling milk production and metabolic pathways in which these genes are involved. With |FC|>1.4, and p-value≤0.05, 142 and 14 genes resulted differentially expressed in stages 01 and 02, respectively. Thu, 07 Apr 2011 22:00:00 GMT http://hdl.handle.net/2067/1893 2011-04-07T22:00:00Z http://hdl.handle.net/2067/1842 Title: Proteomics and Transcriptomics Investigation on longissimus Muscles in Large White and Casertana Pig Breeds Authors: Murgiano, Leonardo; D'Alessandro, Angelo; Egidi, Maria Giulia; Crisa, Alessandra; Prosperini, Gianluca; Timperio, Anna Maria; Zolla, Lello Abstract: Consumer complaints against the blandness of modern lean meat and the frequent reference to the more strongly flavored meat that was available years ago have prompted reconsideration of high fatdepositing typical pig breeds. Casertana and Large White pig breeds are characterized by a different tendency toward fat accumulation as they exhibit opposite genetic and physiological traits with respect to the energy metabolism. These physiological differences were investigated in longissimus lumborum muscles through proteomics (2-DE, MS/MS) and microarray approaches. Data were analyzed for pathway and network analyses, as well as GO term enrichment of biological functions. As a result, Casertana showed a greater amount of proteins involved in glycolitic metabolism and mainly rely on fast-mobilizable energy sources. Large White overexpressed cell cycle and skeletal muscle growth related genes. Metabolic behavior and other implications are discussed. Description: L'articolo è disponibile sul sito dell'editore http://pubs.acs.org/ Thu, 31 Dec 2009 23:00:00 GMT http://hdl.handle.net/2067/1842 2009-12-31T23:00:00Z http://hdl.handle.net/2067/1889 Title: A Tool for Sheep Product Quality: Custom Microarrays from Public Databases Authors: Bongiorni, Silvia; Chillemi, Giovanni; Prosperini, Gianluca; Bueno, Susana; Valentini, Alessio; Pariset, Lorraine Abstract: Milk and dairy products are an essential food and an economic resource in many countries. Milk component synthesis and secretion by the mammary gland involve expression of a large number of genes whose nutritional regulation remains poorly defined. The purpose of this study was to gain an understanding of the genomic influence on milk quality and synthesis by comparing two sheep breeds with different milking attitude (Sarda and Gentile di Puglia) using sheep-specific microarray technology. From sheep ESTs deposited at NCBI, we have generated the first annotated microarray developed for sheep with a coverage of most of the genome. Tue, 05 Apr 2011 22:00:00 GMT http://hdl.handle.net/2067/1889 2011-04-05T22:00:00Z