http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Fri, 24 May 2013 05:29:04 GMT 2013-05-24T05:29:04Z http://hdl.handle.net/2067/1842 Title: Proteomics and Transcriptomics Investigation on longissimus Muscles in Large White and Casertana Pig Breeds Authors: Murgiano, Leonardo; D'Alessandro, Angelo; Egidi, Maria Giulia; Crisa, Alessandra; Prosperini, Gianluca; Timperio, Anna Maria; Zolla, Lello Abstract: Consumer complaints against the blandness of modern lean meat and the frequent reference to the more strongly flavored meat that was available years ago have prompted reconsideration of high fatdepositing typical pig breeds. Casertana and Large White pig breeds are characterized by a different tendency toward fat accumulation as they exhibit opposite genetic and physiological traits with respect to the energy metabolism. These physiological differences were investigated in longissimus lumborum muscles through proteomics (2-DE, MS/MS) and microarray approaches. Data were analyzed for pathway and network analyses, as well as GO term enrichment of biological functions. As a result, Casertana showed a greater amount of proteins involved in glycolitic metabolism and mainly rely on fast-mobilizable energy sources. Large White overexpressed cell cycle and skeletal muscle growth related genes. Metabolic behavior and other implications are discussed. Description: L'articolo è disponibile sul sito dell'editore http://pubs.acs.org/ Thu, 31 Dec 2009 23:00:00 GMT http://hdl.handle.net/2067/1842 2009-12-31T23:00:00Z http://hdl.handle.net/2067/1880 Title: Comparison of Milk Fat Globule Membrane (MFGM) Proteins of Chianina and Holstein Cattle Breed Milk Samples Through Proteomics Methods Authors: Murgiano, Leonardo; Timperio, Anna Maria; Zolla, Lello; Bongiorni, Silvia; Valentini, Alessio; Pariset, Lorraine Abstract: Identification of proteins involved in milk production is important to understand the biology of lactation. Many studies have advanced the understanding of mammary function and milk secretion, but the critical molecular mechanisms implicated in milk fat secretion is still incomplete. Milk Fat Globules are secreted from the apical surface of the mammary cells, surrounded by a thin membrane bilayer, the Milk Fat Globule Membrane (MFGM), formed by proteins which have been suggested to be holesterolemia-lowering factors, inhibitors of cancer cell growth, vitamin binders, bactericidal, suppressors of multiple sclerosis. Using a proteomic approach, we compared MFGM from milk samples of individuals belonging to two different cattle breeds, Chianina and Holstein,representative of selection for milk and meat traits, respectively. We were able to isolate some of the major MFGM proteins in the examined samples and to identify differences between the protein fractions of the two breeds. We detected differences in the amount of proteins linked to mammary gland development and lipid droplets formation, as well as host defence mechanisms. We have shown that proteomics is a suitable, unbiased method for the study of milk fractions proteins and a powerful tool in nutritional genomics. Tue, 05 Apr 2011 22:00:00 GMT http://hdl.handle.net/2067/1880 2011-04-05T22:00:00Z