http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Sat, 25 May 2013 08:46:39 GMT 2013-05-25T08:46:39Z http://hdl.handle.net/2067/1352 Title: Low molecular weight polyethylene glycol induces chromosome aberrations in Chinese hamster ovary (CHO) cells. Authors: Biondi, Olga; Motta, Salvatore; Mosesso, Pasquale Abstract: The human population is widely exposed to polyethylene glycol (PEG) and its chemical derivatives, which are widely used as vehicles or co-solvents in many pharmaceutical and cosmetic preparations. However, PEG polymers of low molecular weight differ significantly from polymers of higher molecular weight in their physico-chemical properties, biological effects on cell permeability and their absorption and excretion, as well as their higher toxicity and possibly genotoxicity. In the present study we have analysed the induction of chromosome aberrations by the low molecular weight PEG polymers tetraethylene glycol (TEG), PEG 200 and PEG 400 in a Chinese hamster epithelial liver (CHEL) cell line, which retains sufficient metabolic capability to activate different promutagens and procarcinogens. The results indicate that in CHEL cells only TEG and PEG 200 are clastogenic. Parallel experiments performed in CHO cells in the presence and absence of rat liver S9 mix showed significant increases in chromosomal aberrations only in cultures treated with TEG in the presence of rat liver S9, indicating that low molecular weight polymers need to be activated to exert their genotoxic activity. Description: L'articolo è disponibile sul sito dell'editore: http://www.oxfordjournals.org Mon, 31 Dec 2001 23:00:00 GMT http://hdl.handle.net/2067/1352 2001-12-31T23:00:00Z http://hdl.handle.net/2067/1351 Title: Cytogenetic evaluation of extractable agents from airborne particulate matter generated in the city of Catania (Italy) Authors: Motta, Salvatore; Federico, Concetta; Saccone, Salvatore; Librando, Vito; Mosesso, Pasquale Abstract: In order to document cytogenetic damage associated with air pollution and, possibly, with health effects in the city of Catania, Sicily (Italy), we analyzed the induction of chromosomal aberrations by extractable agents from airborne particulate matter in a Chinese hamster epithelial liver (CHEL) cells. These cells retain their metabolic competence to activate different classes of promutagens/procarcinogens into biologically active metabolites. Airborne particulate matter was obtained from two stationary samplers (stations I and II) in two areas endowed by an elevated car transit in the centre of Catania. The results obtained clearly indicated that airborne particulate matter from both stations I and II proved to be clastogens in CHEL cells but not in Chinese hamster ovary (CHO) cells without metabolic activation, indicating that airborne particulate mixtures need to be metabolically converted before exerting their genotoxic potential. On the basis of these results we can assert that the test system employed to identify the cytogenetic potential of airborne particulate matter is useful and profitable for environmental control, and helpful to plan specific actions aimed at reducing the hazards derived from exposure to polluted air. Description: L'articolo è disponibile sul sito dell'editore: http://www.sciencedirect.com Wed, 31 Dec 2003 23:00:00 GMT http://hdl.handle.net/2067/1351 2003-12-31T23:00:00Z