http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Sat, 18 May 2013 20:04:27 GMT 2013-05-18T20:04:27Z http://hdl.handle.net/2067/1778 Title: Genetic and cytogenetic maps of durum wheat-Thinopyrum ponticum recombinant chromosomes: a tool to finely describe Triticeae 7L arms and to target alien traits. Authors: Gennaro, Andrea; Kuzmanović, Ljiljana; Forte, Paola; Lattanzi, Gionata; Grossi, Rosaria; Bitti, Alessandra; Giorgi, Debora; Ceoloni, Carla Abstract: As one important result of wheat chromosome engineering, creation of arrays of wheat-alien recombinant chromosomes allows assignment of markers and genes to defined physical locations, hence improving knowledge of structural-functional organization of wheat and alien chromosomal regions. In this research we have exploited several durum wheat-Thinopyrum ponticum recombinant lines carrying relevant genes for wheat improvement. Thu, 31 Dec 2009 23:00:00 GMT http://hdl.handle.net/2067/1778 2009-12-31T23:00:00Z http://hdl.handle.net/2067/1855 Title: Cytogenetic and molecular characterization of durum wheat chromosome transfers with 1D-associated gluten protein genes and their pyramiding Authors: Gennaro, Andrea; Forte, Paola; Lattanzi, Gionata; Ferri, Daniela; Carozza, Roberta; D'Egidio, Maria Grazia; Lafiandra, Domenico; Ceoloni, Carla Abstract: Gluten quality of bread wheat is known to be mainly associated with high- (HMW-GS) and low-(LMW-GS) molecular weight glutenin subunits encoded by Glu-1 (L arm of group-1 chromosomes) and Glu-3 (S arm of group-1 chromosomes) genes, respectively, with the 1D alleles of such genes having the major impact on bread making properties. Transfer of chromosomal segments containing the Glu-D1 and Gli-D1/Glu-D3 loci was successfully achieved in a number of instances resorting to chromosome engineering. Using this strategy, we isolated two 1A-1D recombinant lines, in which the Gli-D1/Glu-D3 genes and the Glu-D1d allele (HMW-GS "5+10") were separately transferred into the 1AS and 1AL arm, respectively, of recipient durum wheat lines (named PS and PL, respectively). Also, a detailed genetic map of both recombinant chromosome arms was developed. Stable PS + PL double-recombinant lines have been obtained as a result of homologous recombination in the 1A portions shared by the two recombinant chromosomes present in PS x PL hybrids. Preliminary quality tests suggest that the Glu-D3 + Glu-D1d combined presence could determine a slight increase of gluten quality parameters over those associated with Glu-D1d alone. Wed, 31 Dec 2008 23:00:00 GMT http://hdl.handle.net/2067/1855 2008-12-31T23:00:00Z