http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Tue, 21 May 2013 19:09:16 GMT 2013-05-21T19:09:16Z http://hdl.handle.net/2067/1308 Title: The intergenic spacer region of the rDNA of Olea europaea Authors: Maggini, Fabio; Gelati, Maria Teresa; Spolverini, Michela; Frediani, Manuela Abstract: The intergenic spacer (IGS) region of rDNA in Olea europaea is 5,629 bp long and it can be subdivided, on the basis of the different features, into six regions; two of them, located upstream and downstream of the putative transcription initiation site (TIS), respectively, contain prominent and unrelated subrepeats (s.r.). The upstream s.r. are 75–86 bp in length and the downstream ones are about 160 bp in length. The first type is represented by a high number (36) of iterations, which share a mutual similarity of 82.5%. A single sequence (TATTATAGGGGGGAGG) is found, which fits the reported TIS of plants and the position +1 would correspond to the adenine at position 3,622 after the 3′ end of 25 S ribosomal RNA (rDNA) genes. Moreover, in Olea IGS region some unusual features are observed and discussed in relation to analogous data present in the literature. Description: L'articolo é disponibile sul sito dell'editore: http://www.springerlink.com Mon, 31 Dec 2007 23:00:00 GMT http://hdl.handle.net/2067/1308 2007-12-31T23:00:00Z http://hdl.handle.net/2067/1309 Title: Phylogenetic relationships among annual and perennial species of the genus Cicer as inferred from ITS sequences of nuclear ribosomal DNA Authors: Frediani, Manuela; Caputo, Paolo Abstract: The cladistic analysis of the DNA sequences of the internal transcribed spacers of ribosomal cistrons (ITS1 and ITS2) for 20 species of Cicer L. (among which all the annuals), shows that various sections of the genus are not monophyletic. Annual species do not form a clade: C. arietinum, in fact, is closely related to both C. echinospermum and C. reticulatum, whereas C. bijugum, C. judaicum, and C. pinnatifidum form a separate clade. The annual C. cuneatum is sister group to the perennial C. canariense and both are archaic species within the genus. C. yamashitae is, on the contrary, the only annual species belonging to a group of perennials, within which close relationships are evident between C. graecum and C. montbretii as well as among a group of mainly Asian species. Description: L'articolo é disponibile sul sito dell'editore: http://www.springerlink.com Fri, 31 Dec 2004 23:00:00 GMT http://hdl.handle.net/2067/1309 2004-12-31T23:00:00Z http://hdl.handle.net/2067/1537 Title: Cytological and molecular characterization of Vicia esdraelonensis Warb. & Eig: a rare taxon Authors: Ruffini Castiglione, Monica; Frediani, Manuela; Gelati, Maria Teresa; Ravalli, Concetta; Venora, Gianfranco; Caputo, Paolo; Cremonini, Roberto Description: L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com Sun, 31 Dec 2006 23:00:00 GMT http://hdl.handle.net/2067/1537 2006-12-31T23:00:00Z http://hdl.handle.net/2067/1540 Title: Cytological characterization of Vicia oroboides Wulfen in Jacq Authors: Ruffini Castiglione, Monica; Frediani, Manuela; Ravalli, Concetta; Venora, Gianfranco; Cremonini, Roberto Description: L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com Wed, 31 Dec 2008 23:00:00 GMT http://hdl.handle.net/2067/1540 2008-12-31T23:00:00Z http://hdl.handle.net/2067/1541 Title: Cytology of Vicia species. X. Karyotype evolution and phylogenetic implication in Vicia species of the sections Atossa, Microcarinae, Wiggersia and Vicia Authors: Ruffini Castiglione, Monica; Frediani, Manuela; Gelati, Maria Teresa; Ravalli, Concetta; Venora, Gianfranco; Caputo, Paolo; Cremonini, Roberto Abstract: Automated karyotype analyses and sequence of rDNA spacers have been analysed for the species belonging to sections Atossa, Microcarinae, Wiggersia and Vicia. Karyomorphological parameters, based on Rec, Syi and TF % indices, have been determined and evidenced that, in term of symmetry, the karyotype of Vicia lathyroides was the most asymmetric one. A multivariate analysis using 34 karyological parameters, in addition to the symmetry indices, has been carried out and the corresponding dendrogram of linkage distances showed six different groups. Molecular investigations on the inclusive group in study by employing ITS DNA sequences indicated a different pattern of relationships. The cladistic analysis combining the molecular data set with karyological parameters evidenced that the species of sections Vicia and Atossa join closely to each other in a paraphyletic group, which includes the monophyletic section Wiggersia. Therefore, our karyological and molecular data provide information about the phylogenetic position of the analysed species inside the subgenus Vicia and are discussed in relation to previous results obtained by morphology, isozymes and ribosomal genes analyses. Description: L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com Thu, 31 Dec 2009 23:00:00 GMT http://hdl.handle.net/2067/1541 2009-12-31T23:00:00Z http://hdl.handle.net/2067/1538 Title: Cytological investigation on Haplopappus gracilis (Nutt.) Gray: 5-methylcytosine rich-regions, fluorochrome banding and chromatin sensitivity to DNase I digestion Authors: Ruffini Castiglione, Monica; Frediani, Manuela; Venora, Gianfranco; Cremonini, Roberto Description: L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com Mon, 31 Dec 2007 23:00:00 GMT http://hdl.handle.net/2067/1538 2007-12-31T23:00:00Z http://hdl.handle.net/2067/1553 Title: Karyomorphological data as phylogenetic markers in the study of Vicia subgenus Authors: Venora, Gianfranco; Ravalli, Concetta; Ruffini Castiglione, Monica; Cremonini, Roberto; Frediani, Manuela Wed, 31 Dec 2008 23:00:00 GMT http://hdl.handle.net/2067/1553 2008-12-31T23:00:00Z http://hdl.handle.net/2067/1558 Title: Nuclear DNA contents, rDNAs and karyotype evolution in subgenus Vicia. III. The heterogeneous section Hypechusa Authors: Caputo, Paolo; Frediani, Manuela; Venora, Gianfranco; Ravalli, Concetta; Ambrosio, Maria; Cremonini, Roberto Abstract: Nuclear DNA contents, automated karyotype analyses, and sequences of internal transcribed spacers from ribosomal genes have been determined in the species belonging to section Hypechusa of the subgenus Vicia. Karyomorphological results and phylogenetic data generated from the comparison of rDNA (genes coding for rRNA) sequences showed that sect. Hypechusa is not monophyletic; however, some monophyletic units are apparent (one including Vicia galeata, V. hyrcanica, V. noeana, and V. tigridis, another including V. assyriaca, V. hybrida, V. melanops, V. mollis, and V. sericocarpa), which partly correspond to morphology-based infrasectional groups. The relationships among these species and the species in sections Faba, Narbonensis, Bithynicae, and Peregrinae have been also investigated. Description: L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com Sat, 31 Dec 2005 23:00:00 GMT http://hdl.handle.net/2067/1558 2005-12-31T23:00:00Z http://hdl.handle.net/2067/1896 Title: Ampelographic and DNA Characterization of Local Grapevine Accessions of the Tuscia Area (Latium, Centre of Italy) Authors: Muganu, Massimo; Dangl, Gerald; Aradhya, Malli; Frediani, Manuela; Scossa, Angela; Stover, Ed Abstract: The presence of local vines in the Tuscia area (Latium region, Italy) was documented by historical sources. Ampelographic and molecular characterization (35 morphological descriptors and 16 microsatellite loci) was carried out on accessions belonging to the most frequently mentioned local vines. SSR markers have enabled us to distinguish cases of local synonymy and microsatellite profiles have permitted comparison with previously reported profiles indicating synonymy and divergence with vines in other collections. Ampelographic traits showed that some accessions of the same genotype differ in minor characteristics that can have a role in clonal evaluation. Wed, 31 Dec 2008 23:00:00 GMT http://hdl.handle.net/2067/1896 2008-12-31T23:00:00Z