http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Tue, 18 Jun 2013 22:38:44 GMT 2013-06-18T22:38:44Z http://hdl.handle.net/2067/2203 Title: Introgressione criptica di DNA parentale in linee di frumento derivate da ibridazione intergenerica fra Triticum aestivum e Dasypyrum villosum Authors: Caceres, Maria Eugenia; Vaccino, Patrizia; Ceccarelli, Marilena; Sarri, Vania; Polizzi, Enza; De Pace, Ciro; Cionini, Pier Giorgio Abstract: Cytogenetic and DNA molecular analyses have been carried out in three wheat inbred lines (CS V58, CS V59 and CS V60), derived from Triticum aestivum cv. ‘Chinese Spring’ (CS) Dasypyrum villosum (Dv) intergeneric hybridization, which showed several Dv-specific phenotypic characters. All lines were found to share with CS the number (2n=42) and structure of the chromosomes and chromatin from Dv was apparently not added to their complement. However, Feulgen/DNA cytophotometry showed that there was more nuclear DNA in the lines than in the parental wheat (by 1.85%, 2.76% and 1.26% in CS V58, CVS V59 and CS V60, respectively). AFLP analysis of genomic DNA from the lines, CS and Dv detected a total of 120 polymorphic bands, 7 of which (5.8%) were present in both the lines and Dv but absent in CS. PCR amplification using specific primers revealed, in the genome of the lines, Dv alleles and not CS alleles at two loci (Vrn-A1 and Vrn-B3). These results indicate cryptic introgression of Dv DNA sequences into the genome of the lines. Some implications of this finding are briefly discussed. Fri, 31 Dec 2010 23:00:00 GMT http://hdl.handle.net/2067/2203 2010-12-31T23:00:00Z http://hdl.handle.net/2067/2202 Title: Citogenetica di ibridi fra Triticum aestivum e Dasypyrum villosum e di linee da essi derivate Authors: Caceres, Maria Eugenia; Ceccarelli, Marilena; De Pace, Ciro; Cionini, Pier Giorgio Abstract: In the course of a research aimed at the genetic improvement of wheat through intergeneric hybridization, 24 inbred breeding lines obtained by the hybridization of Triticum aestivum cv. ‘Chinese Spring’ (CS) or T. turgidum ssp. durum cv. ‘Modoc’ followed by backcross to CS have been characterized cytogenetically. Some lines were found to have the same karyotype as CS. In other lines, karyological events such as chromosome addictions, chromosome substitutions, recombinations between parental genomes or between the homoeologous genomes of wheat were observed. These findings, which were in part unexpected and even surprising, show that a careful cytogenetic characterization of wheat hybrids is necessary to understand factors and mechanisms that play a role in determining the structure of their karyotype, and then to choose strategies for the genetic improvement of wheat by interspecific and/or intergeneric hybridization. Fri, 31 Dec 2010 23:00:00 GMT http://hdl.handle.net/2067/2202 2010-12-31T23:00:00Z