http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Wed, 19 Jun 2013 18:24:45 GMT 2013-06-19T18:24:45Z http://hdl.handle.net/2067/1836 Title: Pyramiding different alien chromosome segments in durum wheat: feasibility and breeding potential Authors: Gennaro, Andrea; Forte, Paola; Carozza, Roberta; Savo Sardaro, Luisa Maria; Ferri, Daniela; Bitti, Alessandra; Borrelli, Grazia Maria; D'Egidio, Maria Grazia; Ceoloni, Carla Abstract: Wheat chromosome engineering, i.e. the transfer of alien chromosome segments from various Triticeae species into cultivated wheats, is greatly benefiting from the recent advancements in molecular genetics, cytogenetics and genomics. Powerful tools are currently available that make selection of desired genotypes far more precise and effective than in the past, thus giving this transfer strategy considerable potential for meaningful practical achievements. The paper describes the pyramiding of various disease resistance and quality-related genes, originating from different alien species, into durum wheat. Description: L'articolo é disponibile sul sito dell'editore: http://www.sciencefromisrael.com Sun, 31 Dec 2006 23:00:00 GMT http://hdl.handle.net/2067/1836 2006-12-31T23:00:00Z http://hdl.handle.net/2067/1855 Title: Cytogenetic and molecular characterization of durum wheat chromosome transfers with 1D-associated gluten protein genes and their pyramiding Authors: Gennaro, Andrea; Forte, Paola; Lattanzi, Gionata; Ferri, Daniela; Carozza, Roberta; D'Egidio, Maria Grazia; Lafiandra, Domenico; Ceoloni, Carla Abstract: Gluten quality of bread wheat is known to be mainly associated with high- (HMW-GS) and low-(LMW-GS) molecular weight glutenin subunits encoded by Glu-1 (L arm of group-1 chromosomes) and Glu-3 (S arm of group-1 chromosomes) genes, respectively, with the 1D alleles of such genes having the major impact on bread making properties. Transfer of chromosomal segments containing the Glu-D1 and Gli-D1/Glu-D3 loci was successfully achieved in a number of instances resorting to chromosome engineering. Using this strategy, we isolated two 1A-1D recombinant lines, in which the Gli-D1/Glu-D3 genes and the Glu-D1d allele (HMW-GS "5+10") were separately transferred into the 1AS and 1AL arm, respectively, of recipient durum wheat lines (named PS and PL, respectively). Also, a detailed genetic map of both recombinant chromosome arms was developed. Stable PS + PL double-recombinant lines have been obtained as a result of homologous recombination in the 1A portions shared by the two recombinant chromosomes present in PS x PL hybrids. Preliminary quality tests suggest that the Glu-D3 + Glu-D1d combined presence could determine a slight increase of gluten quality parameters over those associated with Glu-D1d alone. Wed, 31 Dec 2008 23:00:00 GMT http://hdl.handle.net/2067/1855 2008-12-31T23:00:00Z