http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Sat, 18 May 2013 21:18:24 GMT 2013-05-18T21:18:24Z http://hdl.handle.net/2067/1685 Title: Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus Authors: Scapigliati, Giuseppe; Buonocore, Francesco; Randelli, Elisa; Casani, Daniela; Meloni, Sabrina; Zarletti, Gianpaolo; Tiberi, M.; Pietretti, D.; Boschi, I.; Manchado, Manuel; Martin-Antonio, B.; Jimenez-Cantizano, R.; Bovo, Giuseppe; Borghesan, Fabio; Lorenzen, Niels; Einer-Jensen, Katja; Adams, S.; Thompson, K.; Alonso, C.; Béjar, Julia; Cano, Irene; Borrego, Juan J.; Alvarez, M.Carmen Abstract: Naïve sea bass juveniles (38.4 ± 4.5 g) were intraperitoneally infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6 %. At various intervals, sampling of fish tissues were performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: IFN-1, Mx, IL-1, Cox-2; IL-10, TGF-, TCR, CD4, CD8, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable in “vitro” increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for IFN-1, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF- and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus. Description: L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com/ Fri, 31 Dec 2010 23:00:00 GMT http://hdl.handle.net/2067/1685 2010-12-31T23:00:00Z