http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Sat, 25 May 2013 16:09:41 GMT 2013-05-25T16:09:41Z http://hdl.handle.net/2067/1893 Title: Transcriptomic analysis of two sheep breeds during lactation, using a new custom microarray platform Authors: Bongiorni, Silvia; Chillemi, Giovanni; Prosperini, Gianluca; Bueno, Susana; Signorelli, Federica; Moioli, Bianca; Pariset, Lorraine Abstract: We aim at understanding the genomic influence on milk quality and synthesis by comparing two sheep breeds using sheep-specific microarray technology. From sheep ESTs deposited at NCBI we generated a chip carrying about 22,000 non-redundant features in quadruplicate, achieving very good technical outcomes. Oligos were in situ generated on chip using the Combimatrix equipment. We analysed the mammary transcriptome in individuals of two sheep breeds at two lactation stages, to identify genes controlling milk production and metabolic pathways in which these genes are involved. With |FC|>1.4, and p-value≤0.05, 142 and 14 genes resulted differentially expressed in stages 01 and 02, respectively. Thu, 07 Apr 2011 22:00:00 GMT http://hdl.handle.net/2067/1893 2011-04-07T22:00:00Z http://hdl.handle.net/2067/1878 Title: Not working on mice or humans Authors: Pariset, Lorraine; Bueno, Susana; Bongiorni, Silvia; Valentini, Alessio Abstract: Microarrays technology is fast devel- oping and its application is expanding from Homo sapiens to a wide number of species where enough information on sequences and annotations are available. The number of species for which a dedicated platform exists is not high. The Expressed Sequence Tags (ESTs) databases represent a collection of anonymous sequences that can be used to build species spe- cific microarrays for species whose genome sequences are largely un- known. Tue, 05 Apr 2011 22:00:00 GMT http://hdl.handle.net/2067/1878 2011-04-05T22:00:00Z http://hdl.handle.net/2067/1889 Title: A Tool for Sheep Product Quality: Custom Microarrays from Public Databases Authors: Bongiorni, Silvia; Chillemi, Giovanni; Prosperini, Gianluca; Bueno, Susana; Valentini, Alessio; Pariset, Lorraine Abstract: Milk and dairy products are an essential food and an economic resource in many countries. Milk component synthesis and secretion by the mammary gland involve expression of a large number of genes whose nutritional regulation remains poorly defined. The purpose of this study was to gain an understanding of the genomic influence on milk quality and synthesis by comparing two sheep breeds with different milking attitude (Sarda and Gentile di Puglia) using sheep-specific microarray technology. From sheep ESTs deposited at NCBI, we have generated the first annotated microarray developed for sheep with a coverage of most of the genome. Tue, 05 Apr 2011 22:00:00 GMT http://hdl.handle.net/2067/1889 2011-04-05T22:00:00Z