http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. Wed, 22 May 2013 08:01:52 GMT 2013-05-22T08:01:52Z http://hdl.handle.net/2067/1073 Title: Molecular approach to the study of hemocyanin in stoneflies (Plecoptera): a biologic and phylogenetic interpretation Authors: Amore, Valentina Abstract: Contrary to what was assumed regarding the presence of respiratory proteins in insects, a functional hemocyanin was recently found in larvae and adults of the stoneflies species Perla marginata in P. grandis. In order to verify if the presence of this ancient trait is widespread within the order and to investigate why stoneflies have maintained it, we have extended the search for hemocyanin within Plecoptera families. In particular, we assessed the presence of hemocyanin in the larval and adult stage the seven families of the European stonefly fauna, and we tested some taxa of Oriental and Southern African fauna living in environments with different ecological features respect to those of the Palaearctic streams. We cloned and sequenced cDNAs corresponding to the conserved hemocyanin fragment (in domain II) and studied their expression with PCR technique. To evaluate hemocyanin presence as translated product, highly sensitive method based on liquid chromatography tandem mass spectrometry, was used. Phylogenetic analyses were inferred. On the basis of our outcomes, we have revealed that hemocyanin seems to be not uniformly distributed within this order. Phylogenetic relations, environmental induction and biological aspects, such as larval size, life cycle length and trophic roles, are discussed as possible factors that may be correlated with the presence or absence of hemocyanin in the studied species. Moreover, we take into account the potential multifunctionality of hemocyanin. In this work we presented the current knowledge about the hemocyanin in the Plecoptera as outcomes of our research.; Diversamente dalle conoscenze attuali sulla presenza delle proteine respiratorie negli insetti, è stata recentemente ritrovata emocianina in larve ed adulti delle specie di plecotteri Perla marginata in P. grandis. Allo scopo di verificare se la presenza di questo carattere antico è diffuso all’interno dell’ordine e per indagare sulle motivazione per cui i plecotteri lo hanno mantenuto, abbiamo esteso la ricerca dell’emocianina all’interno di questo gruppo. In special modo, abbiamo comprovato la presenza in larve ed adulti nelle sette famiglie della fauna europea, ed inoltre abbiamo saggiato qualche taxa della fauna orientale e sudafricana, caratterizzate da condizioni ecologiche differenti rispetto a quelle dei fiumi paleartici. Abbiamo clonato e sequenziato cDNA corrispondenti al frammento conservato di emocianina (nel dminio II), ed abbiamo studiato la loro espressione con tecniche di PCR. Per accertare la presenza di emocianina come prodotto di traduzione, si è utilizzato un metodo altamente sensibile basato sulla spettrometria di massa. Infine la presenza dell’emocianina è stata analizzata in chiave filogenetica. Sulla base dei risultati ottenuti, abbiamo messo evidenza che l’emocianina sembra non essere uniformemente distribuita all’interno dell’ordine. Le relazioni filogenetiche, l’induzione ambientale ed alcuni caratteri biologici, come la grandezza delle larve, la durata del ciclo biologico ed il ruolo trofico, sono state discusse come possibili fattori che possono essere correlati con la presenza o l’assenza dell’emocianina nelle specie studiate. Inoltre sono state considerati le possibili multifunzioni dell’emocianina. Nel presente lavoro viene raccolto lo stato di attuale conoscenza sul tema dell’emocianina nei plecotteri, frutto di questa ricerca. Description: Dottorato di ricerca in Ecologia e gestione delle risorse biologiche Sun, 14 Mar 2010 23:00:00 GMT http://hdl.handle.net/2067/1073 2010-03-14T23:00:00Z http://hdl.handle.net/2067/1452 Title: New data on the presence of hemocyanin in Plecoptera: recomposing a puzzle Authors: Amore, Valentina; Gaetani, Brunella; Puig, Maria Ángels; Fochetti, Romolo Fri, 31 Dec 2010 23:00:00 GMT http://hdl.handle.net/2067/1452 2010-12-31T23:00:00Z http://hdl.handle.net/2067/1426 Title: Do all stoneflies nymphs have respiratory proteins? Further data on the presence of hemocyanin in the larval stages of plecoptera species Authors: Amore, Valentina; Belardinelli, Maria Cristina; Guerra, Laura; Buonocore, Francesco; Fausto, Anna Maria; Fochetti, Romolo Description: L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com Wed, 31 Dec 2008 23:00:00 GMT http://hdl.handle.net/2067/1426 2008-12-31T23:00:00Z http://hdl.handle.net/2067/1417 Title: Lack of Hemocyanin in Oriental Plecoptera and Multifuncionality of the Protein in Larvae Authors: Fochetti, Romolo; Amore, Valentina; Gaetani, Brunella Fri, 31 Dec 2010 23:00:00 GMT http://hdl.handle.net/2067/1417 2010-12-31T23:00:00Z http://hdl.handle.net/2067/1415 Title: Comparative Proteomic Analysis of Hemocyanins in Dinocras cephalotes and Perla marginata (Plecoptera) Authors: Fochetti, Romolo; Amore, Valentina; Ubero-Pascal, Nicolás; Egidi, Maria Giulia; Timperio, Anna Maria Abstract: Hemocyanins are large oligomeric respiratory proteins found in many arthropods and mollusks. The overall expression of hemocyanin mRNA, revealed by studies on Plecoptera hemocyanin sequencing, has raised the question of whether the protein is expressed or not. In fact, the presence of expressed hemocyanin has only been reported in the literature for one species, Perla marginata (Panzer, 1799). In this paper, we report the presence of hemocyanin and hexamerin proteins in Dinocras cephalotes (Curtis, 1827), a species closely related to P. marginata. To assess the presence of hemocyanin, we used a reproducible and highly sensitive method based on liquid chromatography tandem mass spectrometry. We conclude that regardless of its putative function (respiratory, immune defense, storage protein), the hemocyanin is actually expressed in species in which its mRNA is present. Description: L'articolo è disponibile sul sito dell'editore http://www.entsoc.org Fri, 31 Dec 2010 23:00:00 GMT http://hdl.handle.net/2067/1415 2010-12-31T23:00:00Z http://hdl.handle.net/2067/1445 Title: Biodiversitat de plecòpters i efemeròpters al Parc Nacional d'Aigüestortes i Estany de Sant Maurici Authors: Puig, Maria Ángels; Amore, Valentina; Ubero-Pascal, Nicolás; Fochetti, Romolo Thu, 31 Dec 2009 23:00:00 GMT http://hdl.handle.net/2067/1445 2009-12-31T23:00:00Z http://hdl.handle.net/2067/1418 Title: Present knowledge on the presence of hemocyanin in stoneflies (Insecta: Plecoptera) Authors: Fochetti, Romolo; Amore, Valentina Abstract: Hemocyanin is a respiratory protein that occurs in the main lineages of Arthropoda. In insects hemocyanin is presently known in many orders. Recently, a functional hemocyanin has also been found in the Plecoptera. Further studies have revealed that hemocyanin seems to be not uniformly distributed within this order. In this paper we report additional data, obtained with RT-PCR sequencing, on the presence of hemocyanin in different stonefly species. In addition, we summarise the present knowledge about the distribution of hemocyanin in the Plecoptera. Biological aspects such as larval size, life cycle length, trophic roles and environmental induction are discussed as possible factors that may be correlated with the presence or absence of hemocyanin in the studied species. Description: L'articolo è disponibile sul sito dell'editore http://www.tandf.co.uk/journals/default.asp Wed, 31 Dec 2008 23:00:00 GMT http://hdl.handle.net/2067/1418 2008-12-31T23:00:00Z