http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. 2013-05-20T00:41:17Z http://hdl.handle.net/2067/1763 Title: Flowering earliness in wheat inbred breeding lines derived from T. aestivum ‘Chinese Spring’ x Dasypyrum villosum hybridization is not related to allelic variation at the vernalization loci VRN-A1, VRN-B1, and VRN-D1 Authors: Caceres, Maria Eugenia; Vaccino, Patrizia; Corbellini, Maria; Cionini, Pier Giorgio; Sarri, Vania; Polizzi, Enza; Vittori, Doriano; De Pace, Ciro Abstract: Substantial cryptic chromosome mutations or recombinations or gene mutations have occurred during the earlier generations following the hybridization T. aestivum “Chinese Spring” (CS) x Dasypyrum villosum event. Those genomic modifications were transmitted to the progeny plants, from which wheat introgression breeding lines (IBL) were derived. One of the mutations might have affected a flowering-promoting gene at a locus different from VRN-1, causing (under nonvernalizing condition and 13-14-hour daylength) the IBL “CSxV59” and the F1 hybrids “CSxV59 x cv Salgemma" and “CSxV59 x cv Isengrain” to start anthesis in less than 50 days from sowing compared to CS and to the two parental winter bread wheat cv Salgemma and cv Isengrain, which flowered about 150 days later. 2007-12-31T23:00:00Z http://hdl.handle.net/2067/2203 Title: Introgressione criptica di DNA parentale in linee di frumento derivate da ibridazione intergenerica fra Triticum aestivum e Dasypyrum villosum Authors: Caceres, Maria Eugenia; Vaccino, Patrizia; Ceccarelli, Marilena; Sarri, Vania; Polizzi, Enza; De Pace, Ciro; Cionini, Pier Giorgio Abstract: Cytogenetic and DNA molecular analyses have been carried out in three wheat inbred lines (CS V58, CS V59 and CS V60), derived from Triticum aestivum cv. ‘Chinese Spring’ (CS) Dasypyrum villosum (Dv) intergeneric hybridization, which showed several Dv-specific phenotypic characters. All lines were found to share with CS the number (2n=42) and structure of the chromosomes and chromatin from Dv was apparently not added to their complement. However, Feulgen/DNA cytophotometry showed that there was more nuclear DNA in the lines than in the parental wheat (by 1.85%, 2.76% and 1.26% in CS V58, CVS V59 and CS V60, respectively). AFLP analysis of genomic DNA from the lines, CS and Dv detected a total of 120 polymorphic bands, 7 of which (5.8%) were present in both the lines and Dv but absent in CS. PCR amplification using specific primers revealed, in the genome of the lines, Dv alleles and not CS alleles at two loci (Vrn-A1 and Vrn-B3). These results indicate cryptic introgression of Dv DNA sequences into the genome of the lines. Some implications of this finding are briefly discussed. 2010-12-31T23:00:00Z