http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. 2013-05-20T19:51:19Z http://hdl.handle.net/2067/1828 Title: Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations Authors: Mendoza-de Gyves, Emilio; Royani, Juwartina Ida; Rugini, Eddo Abstract: Multiple shoots of high quality were produced in vitro from nodal explants of Tectona grandis. An average of about 4 shoots/uninodal explant was obtained within 4 weeks of culture on Murashige and Skoog’s (mMS) medium modified by 50% reduction in NH4NO3 concentration, supplemented with benzylaminopurine (1.5 mg L−1); indole-3-butyric acid (0.01 mg L−1) and gibberellic acid (0.1 mg L−1). The latter was applied bothin the medium and by soaking the nodal segments for 10 s. in a gibberellic acid solution of 100 mg L−1. Hundred percent of shoots rooted cultured onmodified MS medium containing IBA (0.5 mg L−1) and putrescine (160 mg L−1). Putrescine promoted both strong and highly ramified roots and fastgrowing shoots during the rooting phase, conditioning the plantlets for a good survival and quality. Plantlets were transferred to jiffy pots for a shortacclimatization stage in greenhouse where they survived at 100%. This highly reproducible procedure can be adopted for large scale teak propagation. Description: L'articolo è disponibile sul sito dell'editore http://www.edpsciences.org 2006-12-31T23:00:00Z http://hdl.handle.net/2067/1653 Title: Accurate and rapic technique for leaf area measurement in medlar (Mespilus germanica L.) Authors: Mendoza-de Gyves, Emilio; Cristofori, Valerio; Fallovo, Carlo; Rouphael, Youssef; Bignami, Cristina Abstract: Accurate measurements of leaf area are important for agronomic and physiological studies. Deter- mining the individual leaf area (A) of medlar (Mespilus germanica L.) involves measurements of leaf parameters, such as length (L) and width (W), or some combinations of these parameters. A two-year investigation was car- ried out in 2005 (on seven genotypes) and in 2006 (on one cultivar) underopen-field conditions to test whether a model could be developed to estimate leaf area across genotypes. Regression analyses of A versus Land Wrevea- led several models could be used for estimating the area of individual medlar leaves. A linear model having LW as the independent variable (A = 1.81 + 0.68 LW) provided the most accurate estimate (R2 = 0.981, MSE = 7) of medlar leaf area. Validation of the model having LWof leaves from another genotype measured in the 2006 expe- riment showed that the correlation between calculated and measured areas was very high. Using the selected model, agronomists and physiologists can accurately and reliably estimate the leaf area of medlarwithout the use of expensive instruments. 2007-12-31T23:00:00Z http://hdl.handle.net/2067/2173 Title: Stimulation of node and lateral shoot formation in micropropagation of olive ( Olea europaea L.) by using dikegulac Authors: Mendoza-de Gyves, Emilio; Mira, Farida Rosana; Ruiu, Fabrizio; Rugini, Eddo Abstract: Dikegulac, a growth regulator, was shown to stimulate in vitro shoot multiplication of olive cultivars Canino, Frantoio, Moraiolo, but not cultivars Rosciola and Piantone di Moiano. The Rugini Olive Medium including zeatin (4.5 lM) was used supplemented with a range of dikegulac concentrations (0–133.4 lM). An optimal result in number of shoots and nodes was obtained on cultivars Canino, Frantoio and Moraiolo at 66.7 lM dikegulac. Higher concentrations did not stimulate additional shoot and node formation and resulted in a drastic reduction in height of shoots. Elongated shoots were rooted and acclimatised and showed normal development compared to control plants. Description: L'articolo è disponibile sul sito dell'editore http://www.springerlink.com 2007-12-31T23:00:00Z http://hdl.handle.net/2067/2175 Title: Micropropagation of Teucrium fruticans L., an ornamental and medicinal plant Authors: Frabetti, Marco; Gutiérrez-Pesce, Patricia; Mendoza-de Gyves, Emilio; Rugini, Eddo Abstract: An efficient protocol for in vitro propagation of the valuable ornamental and medicinal plant Bush germander (Teucrium fruticans L.) was developed through axillary shoot proliferation. A Murashige and Skoog agar medium supplemented with benzylaminopurine (6.6 μM), α-naphthaleneacetic acid (0.053 μM), and sucrose (3%) significantly improved the production of multiple shoots directly from nodal segment explants, resulting in an average of 2.8 shoots per segment with an average of 6.8 nodes per shoot that would be potential newly formed explants. The new shoots were developed without a marked decrease in the average height of the shoots. Shoots treated with 2.5 μM indole-3-butyric acid showed the highest average root number (7.9) and the highest percentage of rooting (94%). Plantlets were hardened off and transferred to jiffy pots for acclimatization under greenhouse conditions, resulting in a 100% survival rate. Description: L'articolo è disponibile sul sito dell'editore http://www.springerlink.com 2008-12-31T23:00:00Z http://hdl.handle.net/2067/2169 Title: Teak Authors: Mendoza-de Gyves, Emilio; Rugini, Eddo Abstract: Teak (Tectona grandis Linn f.) is one of the world's premier hardwood timbers, possessing excellent properties. It has been extensively planted within Asia, its natural range, and throughout the tropical regions of the world, including Pacific, Africa, and Latin America. Teak breeding programs started with selection of superior phenotypes (plus trees) in both natural and established plantations. These selections were made based mainly on their phenotypic appearance. Some help was obtained from the molecular marker identification but only in the last 15 years. Conventional breeding methods have shown to have certain limitations in this species, including long rotation age, difficulties in performing controlled pollination, low germination percentage, and low genetic variability of the species. These limitations make genetic engineering an important tool. Despite the progress made in the last 20 years by using plant molecular approaches to improve some forest trees, the success of teak transformation has been limited. Although some achievements have been made (Agrobacterium susceptibility in teak was tested; the first experiments with transient expression were carried out successfully; identification of genetic markers has been initiated with some progress), the low capacity to regenerate from somatic tissues has obstructed the continuity in transgenic experiments. This limitation has reduced the capacity of the whole transformation process. It is expected to overcome this barrier in order to improve the transformation efficiency. Description: L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com 2007-12-31T23:00:00Z