http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. 2013-05-23T01:08:42Z http://hdl.handle.net/2067/2168 Title: Kiwifruit Authors: Gutiérrez-Pesce, Patricia; Rugini, Eddo Abstract: The wide genetic variation among the different species belonging to the genus Actinidia provides the opportunity for developing new cultivars in order to increase the kiwifruit world market variability, which has been limited to few types. The main expected characteristics for new cultivars are the improvement of fruit quality characteristics, abiotic and biotic stress tolerance, regulation of fruit ripening, the production of hermaphrodite plants, parthenocarpic fruits, and the alteration of vegetative habit. Genetic improvement programs are focused on modifying the expression of the endogenous genes, or on transferring foreign genes through both direct and indirect gene transfer methods. Efficient in vitro regeneration systems are indispensable for the recovery of transgenic plants. Optimal results have been obtained in three species of the Actinidia genus because kiwifruit has demonstrated relative amenability to in vitro multiplication; protoplast culture, somatic hybridization, embryo rescue, and the creation of dihaploids lines have also been developed as methods for genetic improvement. Transgenic plants of A. deliciosa have been recovered with some foreign genes of interest: rolABC, rolB, whole T-DNA, osmotin, human epidermal growth factor, soybean -1,3-endoglucanase, and OSH1. Some of these transgenic plants are under field evaluation for biotic stress resistance and growth habit. Description: L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com 2007-12-31T23:00:00Z http://hdl.handle.net/2067/2175 Title: Micropropagation of Teucrium fruticans L., an ornamental and medicinal plant Authors: Frabetti, Marco; Gutiérrez-Pesce, Patricia; Mendoza-de Gyves, Emilio; Rugini, Eddo Abstract: An efficient protocol for in vitro propagation of the valuable ornamental and medicinal plant Bush germander (Teucrium fruticans L.) was developed through axillary shoot proliferation. A Murashige and Skoog agar medium supplemented with benzylaminopurine (6.6 μM), α-naphthaleneacetic acid (0.053 μM), and sucrose (3%) significantly improved the production of multiple shoots directly from nodal segment explants, resulting in an average of 2.8 shoots per segment with an average of 6.8 nodes per shoot that would be potential newly formed explants. The new shoots were developed without a marked decrease in the average height of the shoots. Shoots treated with 2.5 μM indole-3-butyric acid showed the highest average root number (7.9) and the highest percentage of rooting (94%). Plantlets were hardened off and transferred to jiffy pots for acclimatization under greenhouse conditions, resulting in a 100% survival rate. Description: L'articolo è disponibile sul sito dell'editore http://www.springerlink.com 2008-12-31T23:00:00Z http://hdl.handle.net/2067/1768 Title: Micro-methods for genotypic screening of hazelnut (Corylus avellana L.) seeds to accelerate breeding for seed metabolite improvement Authors: Kuzmanović, Ljiljana; Delfini, Maurizio; Rugini, Eddo; Gutiérrez-Pesce, Patricia; De Pace, Ciro Abstract: We used hazelnut full-sib (FS) seed progenies from controlled pollination for efficiency testing of a multi-micro-method procedure comprising: a generalized low reagent-cost and low amount of extracting-tissue protocol for DNA purification and SSR seed genotyping, nuclear magnetic resonance (NMR) detection of analyte spectra for phenotyping metabolite content, and in vitro culture for clonal propagation of the embryo genotype.These micro-methods can be integrated into breeding programs for hazelnut fruit metabolite improvement. Description: L'articolo é disponibile sul sito dell'editore: http://www.ishs.org 2008-12-31T23:00:00Z http://hdl.handle.net/2067/2170 Title: Olive Authors: Rugini, Eddo; Gutiérrez-Pesce, Patricia; Muleo, Rosario Abstract: Biochemical and molecular procedures have been developed for olive genotyping, for taxonomy between cultivated and wild olives, for phylogenetic studies of cultivars, and for identification of usable markers linked to the most important agronomic traits, such as size of the tree, flowering induction, apical dominance, productivity, self-fertility, quantity and quality of oil and secondary products of fruit, biotic and abiotic resistance. These characters could be introgressed into cultivars by conventional breeding or through transgeny. Successful results by classical breeding are difficult to obtain due to the different flower fertility and the long juvenile period of the progenies. The low efficiency of modern genetic improvement techniques suggests that genetic transformation techniques could speed up the development of new genotypes; however, the development of efficient regeneration methods from mature tissue is the limiting factor for applying this technology, in olive. Transgenic olive plants with modified growth habit and putative induced disease resistance are under filed conditions to test their phenotype. Other attempts of genetic improvement such as clonal selection, mutation through -irradiation, protoplast technology, haploid, changes in ploidy level, somaclonal variation, and somatic hybridization are discussed and reviewed. Description: L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com/ 2007-12-31T23:00:00Z http://hdl.handle.net/2067/2200 Title: In vitro microtuberization for simulating the developmental physiology of underground storage organ in Helianthus tuberosus Authors: Gutiérrez-Pesce, Patricia; Bizzarri, Marco; Rugini, Eddo; De Pace, Ciro Abstract: The method described here allows microtuber induction on single nodal explants dissected from in-vitro grown plantlets from tubers of three Helianthus tuberosus clones: Violet de Rennes (VR), Ungheria-3B(CU-3B), and K8-HS142. 2010-12-31T23:00:00Z