http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. 2013-06-20T10:51:42Z http://hdl.handle.net/2067/1308 Title: The intergenic spacer region of the rDNA of Olea europaea Authors: Maggini, Fabio; Gelati, Maria Teresa; Spolverini, Michela; Frediani, Manuela Abstract: The intergenic spacer (IGS) region of rDNA in Olea europaea is 5,629 bp long and it can be subdivided, on the basis of the different features, into six regions; two of them, located upstream and downstream of the putative transcription initiation site (TIS), respectively, contain prominent and unrelated subrepeats (s.r.). The upstream s.r. are 75–86 bp in length and the downstream ones are about 160 bp in length. The first type is represented by a high number (36) of iterations, which share a mutual similarity of 82.5%. A single sequence (TATTATAGGGGGGAGG) is found, which fits the reported TIS of plants and the position +1 would correspond to the adenine at position 3,622 after the 3′ end of 25 S ribosomal RNA (rDNA) genes. Moreover, in Olea IGS region some unusual features are observed and discussed in relation to analogous data present in the literature. Description: L'articolo é disponibile sul sito dell'editore: http://www.springerlink.com 2007-12-31T23:00:00Z http://hdl.handle.net/2067/1537 Title: Cytological and molecular characterization of Vicia esdraelonensis Warb. & Eig: a rare taxon Authors: Ruffini Castiglione, Monica; Frediani, Manuela; Gelati, Maria Teresa; Ravalli, Concetta; Venora, Gianfranco; Caputo, Paolo; Cremonini, Roberto Description: L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com 2006-12-31T23:00:00Z http://hdl.handle.net/2067/1541 Title: Cytology of Vicia species. X. Karyotype evolution and phylogenetic implication in Vicia species of the sections Atossa, Microcarinae, Wiggersia and Vicia Authors: Ruffini Castiglione, Monica; Frediani, Manuela; Gelati, Maria Teresa; Ravalli, Concetta; Venora, Gianfranco; Caputo, Paolo; Cremonini, Roberto Abstract: Automated karyotype analyses and sequence of rDNA spacers have been analysed for the species belonging to sections Atossa, Microcarinae, Wiggersia and Vicia. Karyomorphological parameters, based on Rec, Syi and TF % indices, have been determined and evidenced that, in term of symmetry, the karyotype of Vicia lathyroides was the most asymmetric one. A multivariate analysis using 34 karyological parameters, in addition to the symmetry indices, has been carried out and the corresponding dendrogram of linkage distances showed six different groups. Molecular investigations on the inclusive group in study by employing ITS DNA sequences indicated a different pattern of relationships. The cladistic analysis combining the molecular data set with karyological parameters evidenced that the species of sections Vicia and Atossa join closely to each other in a paraphyletic group, which includes the monophyletic section Wiggersia. Therefore, our karyological and molecular data provide information about the phylogenetic position of the analysed species inside the subgenus Vicia and are discussed in relation to previous results obtained by morphology, isozymes and ribosomal genes analyses. Description: L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com 2009-12-31T23:00:00Z